Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
| Autor: | Gérald Culioli, Eric Chabriere, David Daudé, Laure Plener, Julien Lopez, Nathan Carriot, Sonia Mion, Annick Ortalo-Magné |
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| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323 (MAPIEM), Université de Toulon (UTLN), Gene&GreenTK, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Proteome Bacillus cereus Applied Microbiology and Biotechnology Microbial ecology Mice 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Lactonase 0303 health sciences education.field_of_study [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases biology Environmental microbiology Chemistry QR100-130 Quorum Sensing food and beverages Quorum Quenching [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Metabolome Biotechnology Saccharomyces cerevisiae Population Bacterial Physiological Phenomena Microbiology Article 03 medical and health sciences Bacterial Proteins [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Animals Metabolomics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology education 030304 developmental biology Bacteria Chromobacterium Macrophages Bacteriology Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Quorum sensing biology.protein Chromobacterium violaceum 030217 neurology & neurosurgery |
| Zdroj: | npj Biofilms and Microbiomes npj Biofilms and Microbiomes, 2021, 7 (1), pp.40. ⟨10.1038/s41522-021-00211-w⟩ npj Biofilms and Microbiomes, [London?]: Springer Nature published in partnership with Nanyang Technological University, 2021, 7 (1), pp.40. ⟨10.1038/s41522-021-00211-w⟩ NPJ Biofilms and Microbiomes npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-16 (2021) |
| ISSN: | 2055-5008 |
| Popis: | Quorum sensing (QS) is a communication system used by bacteria to coordinate a wide panel of biological functions in a cell density-dependent manner. The Gram-negative Chromobacterium violaceum has previously been shown to use an acyl-homoserine lactone (AHL)-based QS to regulate various behaviors, including the production of proteases, hydrogen cyanide, or antimicrobial compounds such as violacein. By using combined metabolomic and proteomic approaches, we demonstrated that QS modulates the production of antimicrobial and toxic compounds in C. violaceum ATCC 12472. We provided the first evidence of anisomycin antibiotic production by this strain as well as evidence of its regulation by QS and identified new AHLs produced by C. violaceum ATCC 12472. Furthermore, we demonstrated that targeting AHLs with lactonase leads to major QS disruption yielding significant molecular and phenotypic changes. These modifications resulted in drastic changes in social interactions between C. violaceum and a Gram-positive bacterium (Bacillus cereus), a yeast (Saccharomyces cerevisiae), immune cells (murine macrophages), and an animal model (planarian Schmidtea mediterranea). These results underscored that AHL-based QS plays a key role in the capacity of C. violaceum to interact with micro- and macroorganisms and that quorum quenching can affect microbial population dynamics beyond AHL-producing bacteria and Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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