Ce-based metal-organic frameworks and DNAzyme-assisted recycling as dual signal amplifiers for sensitive electrochemical detection of lipopolysaccharide
| Autor: | Wen-Jun Shen, Ruo Yuan, Yaqin Chai, Ying Zhuo |
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| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Cations Divalent Biomedical Engineering Biophysics Deoxyribozyme Analytical chemistry Metal Nanoparticles Biosensing Techniques 02 engineering and technology 010402 general chemistry Electrochemistry 01 natural sciences Catalysis Limit of Detection Organometallic Compounds Humans Electrodes Detection limit Chemistry fungi Substrate (chemistry) Cerium DNA Catalytic Electrochemical Techniques General Medicine Aptamers Nucleotide 021001 nanoscience & nanotechnology Ascorbic acid Combinatorial chemistry 0104 chemical sciences Zinc Linear range Colloidal gold Electrode Gold 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 83:287-292 |
| ISSN: | 0956-5663 |
| Popis: | In this work, a sensitive electrochemical aptasensor was designed for lipopolysaccharide (LPS) detection based on Ce-based metal-organic frameworks (Ce-MOFs) and Zn(2+) dependent DNAzyme-assisted recycling as dual signal amplifiers. Herein, Ce-MOFs were decorated with gold nanoparticles (AuNPs) to obtain AuNPs/Ce-MOFs, and the resultant AuNPs/Ce-MOFs not only acted as nanocarriers to capture -SH terminated hairpin probes 2 (HP2) for acquiring HP2/AuNPs/Ce-MOFs signal probes, but also as catalysts to catalyze the oxidation of ascorbic acid (AA). In the presence of target LPS, report DNA was released from the prepared duplex DNA and then hybridized with hairpin probes 1 (HP1, which were immobilized on the electrode). With the help of Zn(2+), report DNA could act as Zn(2+) dependent DNAzyme to cleave HP1 circularly. Then a large amount of capture probes were produced on the electrode to combine with HP2/AuNPs/Ce-MOFs signal probes. When the detection solution contained electrochemical substrate of AA, AuNPs/Ce-MOFs could oxide AA to obtain enhanced signal. Under the optimized conditions, this proposed aptasensor for LPS exhibited a low detection limit of 3.3 fg/mL with a wide linear range from 10fg/mL to 100ng/mL. |
| Databáze: | OpenAIRE |
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