New Dual Monoclonal ELISA for Measuring Plasma Osteopontin as a Biomarker Associated with Survival in Prostate Cancer: Clinical Validation and Comparison of Multiple ELISAs
| Autor: | Alan B. Tuck, Nancy Schmidt, Pieter H. Anborgh, Eric Winquist, Russell Hart, Sylvia M. Wilson, Toshimitsu Uede, Shigeyuki Kon, Larry Stitt, Ann F. Chambers, Masahiro Maeda |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Neoplasms Hormone-Dependent medicine.drug_class Clinical Biochemistry Population Enzyme-Linked Immunosorbent Assay Kaplan-Meier Estimate Monoclonal antibody Mice Prostate cancer stomatognathic system Predictive Value of Tests Prostate Biomarkers Tumor medicine Animals Humans Osteopontin education Proportional Hazards Models Mice Inbred BALB C education.field_of_study biology business.industry Biochemistry (medical) Antibodies Monoclonal Prostatic Neoplasms Cancer medicine.disease Peptide Fragments medicine.anatomical_structure Monoclonal Immunology Cancer research biology.protein Antibody business |
| Zdroj: | Clinical Chemistry. 55:895-903 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Background: A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in breast, prostate, and other cancers. Here we describe the clinical validation of a new dual monoclonal (Dual Mono) assay. We compared both assays with 4 assays that recognize defined regions of OPN protein (dual polyclonal systems 5-1, 4-1, 4-3 and polyclonal-monoclonal system 1-3).Methods: OPN sequences recognized by the monoclonal antibodies that make up the Dual Mono ELISA were identified by Pepscan CLIPS™ analysis. Using the 6 ELISAs, we measured OPN in plasma from 66 patients with castration-resistant prostate cancer, and we assessed the ability of each assay to predict patient survival.Results: The assays varied in measured plasma OPN concentrations, with median values ranging from 112 to 1740 μg/L, and ability to predict patient survival. By Cox univariable regression of survival by tertiles of OPN, the Mono/Poly and Dual Mono ELISAs had the highest log-rank χ2 values. After adjustment for risk factors independently associated with survival in our samples, OPN remained associated with survival only for the Mono/Poly and Dual Mono systems.Conclusions: OPN plasma values varied significantly depending on the assay used. Only the Mono/Poly and Dual Mono systems were independently associated with survival in a population of men with castration-resistant prostate cancer. The availability of a clinically validated, dual monoclonal–based ELISA will provide consistent reagents for studies of OPN plasma concentrations in cancer and other pathologies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|