Sphingomonas aquatica sp. nov., isolated from tap water
| Autor: | Minseok Kim, Jung Hun Jo, Wan-Taek Im, Soon-Youl Lee, Myung-Suk Kang, Gyu-Min Choi |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Spermidine Ubiquinone Sphingomonas desiccabilis Biology medicine.disease_cause Sphingomonas 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences RNA Ribosomal 16S Republic of Korea Botany medicine Phylogeny Ecology Evolution Behavior and Systematics Base Composition Strain (chemistry) Sphingomonas oligophenolica Drinking Water Fatty Acids Sequence Analysis DNA General Medicine Sphingomonas hankookensis Ribosomal RNA 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Sphingomonas pruni Sphingomonas asaccharolytica |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:845-850 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001682 |
| Popis: | A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1T, was isolated from tap water in South Korea. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1T grew at 18–42 °C and at pH 6.0–10.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Sphingomonas and is most closely related to the Sphingomonas oligophenolica JCM 12082T (97.2 % similarity), Sphingomonas asaccharolytica NBRC 15499T (96.8 %), Sphingomonas desiccabilis CP1DT (96.8 %), Sphingomonas pruni NBRC 15498T (96.8 %), Sphingomonas hankookensis ODN7T (96.4 %) and Sphingomonas yabuuchiae DSM 14562T (95.8 %). Chemotaxonomic data [major ubiquinone – Q10, major polyamine – homospermidine, major fatty acids – summed feature 8 (C18 : 1ω7c/ω6c), C16 : 0 and C14 : 0 2-OH and presence of sphingoglycolipid] supported the affiliation of the strain to the genus Sphingomonas . The G+C content of genomic DNA was 67.1 mol%. However, low level of DNA–DNA relatedness value between strain W1-2-1T and S. oligophenolica JCM 12082T and the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain W1-2-1T from other Sphingomonas species with validly published names. Therefore, the isolate represents a novel species, for which the name Sphingomonas aquatica sp. nov. (type strain W1-2-1T=KACC 18309T=LMG 28596T) is proposed. |
| Databáze: | OpenAIRE |
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