| Autor: |
Uwe Völker, Raghavendra Palankar, Manuela Gesell-Salazar, Andreas Greinacher, Martin Beer, Clemens Cammann, Maximilian Schindler, Christian Hentschker, Kati Franzke, Nicole Endlich, Leif Steil, Emil Reisinger, Jan Wesche, Stephan Michalik, Florian Siegerist, Alexander Reder, Ulrike Seifert |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.21203/rs.3.rs-736157/v1 |
| Popis: |
Vector-based SARS-CoV-2 vaccines have been associated with vaccine-induced thrombosis with thrombocytopenia syndrome (VITT/TTS), but the causative factors are still unresolved. We comprehensively analyzed ChAdOx1 nCov-19 (AstraZeneca) and Ad26.COV2.S (Johnson & Johnson). ChAdOx1 nCoV-19 contains significant amounts of host cell protein impurities, including functionally active proteasomes, and adenoviral proteins. In Ad26.COV2.S much less impurities were found. Platelet-factor 4 (PF4) formed complexes with ChAdOx1 nCoV-19 constituents, but not with purified virions from ChAdOx1 nCoV-19 or with Ad26.COV2.S. Vascular hyperpermeability was induced by ChAdOx nCoV-19 but not by Ad26.COV2.S.These differences in impurities together with EDTA-induced capillary leakage might contribute to the higher incidence rate of VITT associated with ChAdOx1 nCoV-19 compared to Ad26.COV2.S. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
