Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum
| Autor: | Jérémy Just, Peter M. Rogowsky, Asher Pasha, José Caïus, Aurélie Grimault, Eddi Esteban, Nicholas J. Provart, Nicolas M. Doll, Thomas Widiez, Véronique Brunaud, Gwyneth C. Ingram, Nathalie Depège-Fargeix |
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| Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Violences, Innovations, Politiques, Socialisations et Sports (VIPS2), Le Mans Université (UM)-École normale supérieure - Rennes (ENS Rennes)-Université de Rennes 2 (UR2), University of Toronto, Plant Science and Breeding Division of the Institut National de la Recherche en Agriculture et Alimentation et Environnement (BAP, INRAE, Project SeedCom), Estonian Research Council, French National Research Agency (ANR), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Le Mans Université (UM)-École normale supérieure - Rennes (ENS Rennes), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, INRA BAP, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
0106 biological sciences Transcription Genetic maïs [SDV]Life Sciences [q-bio] Plant Science Biology maize 01 natural sciences Zea mays In Brief Endosperm Transcriptome endosperm 03 medical and health sciences Cell Wall Gene Expression Regulation Plant Aleurone Compartment (development) [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Plant Proteins Regulation of gene expression 0303 health sciences endosperme Cell Death Gene Expression Profiling transcriptomique Gene Expression Regulation Developmental Reproducibility of Results food and beverages Embryo Cell Biology Scutellum Embryonic stem cell Cell biology Up-Regulation 030104 developmental biology Gene Ontology Mutation seed seed albumins 010606 plant biology & botany |
| Zdroj: | The Plant cell The Plant cell, 2020, 32 (4), pp.833-852. ⟨10.1105/tpc.19.00756⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2020, 32 (4), pp.833-852. ⟨10.1105/tpc.19.00756⟩ BioRxiv BioRxiv, 2019, ⟨10.1101/799338⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2019, ⟨10.1105/tpc.19.00756⟩ |
| ISSN: | 1040-4651 1532-298X |
| Popis: | International audience; A population of endosperm cells adjacent to the embryo scutellum shows transcriptomic enrichment in transport functions and is influenced by embryo development.Seeds are complex biological systems comprising three genetically distinct tissues nested one inside another (embryo, endosperm, and maternal tissues). However, the complexity of the kernel makes it difficult to understand intercompartment interactions without access to spatially accurate information. Here, we took advantage of the large size of the maize (Zea mays) kernel to characterize genome-wide expression profiles of tissues at different embryo/endosperm interfaces. Our analysis identifies specific transcriptomic signatures in two interface tissues compared with whole seed compartments: the scutellar aleurone layer and the newly named endosperm adjacent to scutellum (EAS). The EAS, which appears around 9 d after pollination and persists for around 11 d, is confined to one to three endosperm cell layers adjacent to the embryonic scutellum. Its transcriptome is enriched in genes encoding transporters. The absence of the embryo in an embryo specific mutant can alter the expression pattern of EAS marker genes. The detection of cell death in some EAS cells together with an accumulation of crushed cell walls suggests that the EAS is a dynamic zone from which cell layers in contact with the embryo are regularly eliminated and to which additional endosperm cells are recruited as the embryo grows. |
| Databáze: | OpenAIRE |
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