Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum

Autor: Jérémy Just, Peter M. Rogowsky, Asher Pasha, José Caïus, Aurélie Grimault, Eddi Esteban, Nicholas J. Provart, Nicolas M. Doll, Thomas Widiez, Véronique Brunaud, Gwyneth C. Ingram, Nathalie Depège-Fargeix
Přispěvatelé: Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Violences, Innovations, Politiques, Socialisations et Sports (VIPS2), Le Mans Université (UM)-École normale supérieure - Rennes (ENS Rennes)-Université de Rennes 2 (UR2), University of Toronto, Plant Science and Breeding Division of the Institut National de la Recherche en Agriculture et Alimentation et Environnement (BAP, INRAE, Project SeedCom), Estonian Research Council, French National Research Agency (ANR), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Le Mans Université (UM)-École normale supérieure - Rennes (ENS Rennes), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Department of Cell and Systems Biology/Centre for the Analysis of Genome Evolution and Function, INRA BAP, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
0106 biological sciences
Transcription
Genetic
maïs
[SDV]Life Sciences [q-bio]
Plant Science
Biology
maize
01 natural sciences
Zea mays
In Brief
Endosperm
Transcriptome
endosperm
03 medical and health sciences
Cell Wall
Gene Expression Regulation
Plant
Aleurone
Compartment (development)
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Gene
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Plant Proteins
Regulation of gene expression
0303 health sciences
endosperme
Cell Death
Gene Expression Profiling
transcriptomique
Gene Expression Regulation
Developmental
Reproducibility of Results
food and beverages
Embryo
Cell Biology
Scutellum
Embryonic stem cell
Cell biology
Up-Regulation
030104 developmental biology
Gene Ontology
Mutation
seed
seed albumins
010606 plant biology & botany
Zdroj: The Plant cell
The Plant cell, 2020, 32 (4), pp.833-852. ⟨10.1105/tpc.19.00756⟩
The Plant cell, American Society of Plant Biologists (ASPB), 2020, 32 (4), pp.833-852. ⟨10.1105/tpc.19.00756⟩
BioRxiv
BioRxiv, 2019, ⟨10.1101/799338⟩
The Plant cell, American Society of Plant Biologists (ASPB), 2019, ⟨10.1105/tpc.19.00756⟩
ISSN: 1040-4651
1532-298X
Popis: International audience; A population of endosperm cells adjacent to the embryo scutellum shows transcriptomic enrichment in transport functions and is influenced by embryo development.Seeds are complex biological systems comprising three genetically distinct tissues nested one inside another (embryo, endosperm, and maternal tissues). However, the complexity of the kernel makes it difficult to understand intercompartment interactions without access to spatially accurate information. Here, we took advantage of the large size of the maize (Zea mays) kernel to characterize genome-wide expression profiles of tissues at different embryo/endosperm interfaces. Our analysis identifies specific transcriptomic signatures in two interface tissues compared with whole seed compartments: the scutellar aleurone layer and the newly named endosperm adjacent to scutellum (EAS). The EAS, which appears around 9 d after pollination and persists for around 11 d, is confined to one to three endosperm cell layers adjacent to the embryonic scutellum. Its transcriptome is enriched in genes encoding transporters. The absence of the embryo in an embryo specific mutant can alter the expression pattern of EAS marker genes. The detection of cell death in some EAS cells together with an accumulation of crushed cell walls suggests that the EAS is a dynamic zone from which cell layers in contact with the embryo are regularly eliminated and to which additional endosperm cells are recruited as the embryo grows.
Databáze: OpenAIRE
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