Construction of a potato transcriptome map based on the cDNA–AFLP technique
| Autor: | J. I. Ruiz de Galarreta, E. Ritter, I. Sánchez, H.J. van Eck |
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| Rok vydání: | 2008 |
| Předmět: |
genome-wide expression
DNA Complementary Genetic Linkage markers physical map solanum-tuberosum arabidopsis-thaliana rflp maps Genes Plant resistance Transcriptome Laboratorium voor Plantenveredeling Gene Expression Regulation Plant Genetic linkage Complementary DNA Genetics Coding region Gene Solanum tuberosum Polymorphism Genetic biology Gene Expression Profiling EPS-4 linkage maps food and beverages General Medicine biology.organism_classification gene-expression Gene expression profiling Plant Breeding recombination frequencies Genetic marker Solanum Nucleic Acid Amplification Techniques Agronomy and Crop Science Biotechnology |
| Zdroj: | Theoretical and Applied Genetics, 116(7), 1003-1013 Theoretical and Applied Genetics 116 (2008) 7 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s00122-008-0731-5 |
| Popis: | The cDNA¿AFLP technique can be used to monitor differential gene expression, but also for linkage mapping. Extending previous works, we have now constructed an integrated linkage map of the potato transcriptome based on the said technique that has a length of around 800 cM and contains nearly 700 transcriptome derived fragments (TDFs). At the same time, most of these markers are anchored to the bins of a highly saturated reference map in potato, combining in this way the information provided by different marker types. Moreover, we detected and confirmed an elevated degree of allelic fragments with this marker type, which was present in nearly half of all primer combinations and involved around 20% of all fragments. These properties were particularly useful to establish anchor points for integrating the individual parental linkage maps. Comparative expression profiling in different plant materials revealed that only a few additional TDFs were obtained which were specific for mature leaves or tubers compared to the TDFs present in whole in vitro plants. Since TDF markers are derived from coding regions, they generally also represent sequences with a biological function. In four case studies, co-migrating TDFs in different Solanum wild species always represented potential alleles based on elevated homologies among them. Two resistance gene homologs were identified by analysing TDFs, which were co-located with known QTLs. |
| Databáze: | OpenAIRE |
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