Involvement of Androgen-Induced Growth Factor (FGF-8) Gene in Mouse Embryogenesis and Morphogenesis
| Autor: | Tsutomu Nohno, Yasuhiko Kawakami, Hideyo Ohuchi, Sumihare Noji, Hidefumi Yoshioka, Akira Tanaka |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Apical ectodermal ridge
Fibroblast Growth Factor 8 Biophysics Morphogenesis Ectoderm Biology Fibroblast growth factor Biochemistry Mice medicine Animals RNA Messenger Growth Substances Molecular Biology In Situ Hybridization Primitive streak Embryogenesis Brain Gene Expression Regulation Developmental Extremities Cell Biology Blotting Northern Molecular biology Neoplasm Proteins Fibroblast Growth Factors Mice Inbred C57BL Gastrulation medicine.anatomical_structure Face Nasal placode |
| Zdroj: | Biochemical and Biophysical Research Communications. 204:882-888 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.2542 |
| Popis: | Androgen-induced growth factor (AIGF/FGF-8) has purified from a mouse mammary carcinoma cell line and its cDNA cloning revealed that AIGF is the eighth member of the fibroblast growth factor family. To clarify a biological role of FGF-8, Northern blot analysis of various adult tissues was carried out, but no expression was found. However, whole mount in situ hybridization of 7.5- to 14.5-day gestation (E7.5-14.5) embryos revealed six predominant expression domains: [1]primitive streak region (E7.5-9.75); [2]midbrain-hindbrain border (E8.0-10.5); [3]rostral forebrain (E8.0-10.5); [4]limb ectoderm and apical ectodermal ridge (E9.25-13.5); [5]nasal placode and epithelium (E9.5-12.5); and [6]branchial arch ectoderm (E8.5-11.5). The embryonic expression pattern suggests a unique role of FGF-8 in mouse development, especially in gastrulation, brain development, and limb and facial morphogenesis. |
| Databáze: | OpenAIRE |
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