Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission
| Autor: | Sandra Losada, S. González, T. Hermoso, Italo M. Cesari, C. Balzan, Oscar Noya, B. Alarcón de Noya |
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| Rok vydání: | 1995 |
| Předmět: |
Adolescent
medicine.medical_treatment Blotting Western Immunology Antibodies Helminth Enzyme-Linked Immunosorbent Assay Schistosomiasis Praziquantel Serology Epitopes Feces Immune system Antigen medicine Animals Humans Child Parasite Egg Count Ovum Chemotherapy biology Immune Sera Schistosoma mansoni medicine.disease Precipitin biology.organism_classification Virology Schistosomiasis mansoni Antigens Helminth Child Preschool Chronic Disease Electrophoresis Polyacrylamide Gel Parasitology medicine.drug |
| Zdroj: | Parasite Immunology. 17:111-117 |
| ISSN: | 1365-3024 0141-9838 |
| Popis: | SUMMARY In an attempt to identify antigenic molecules from Schistosoma mansoni eggs, a serological study was performed on children of a Venezuelan town (Carabal-leda) in which the transmission of schistosomiasis had been interrupted two years prior to sera sampling. Infected children received treatment with Praziquantel and, based on the disappearance of eggs in the stools plus negativiza-tion of the circumoval precipitin test (COPT) one year after treatment, they were classified as either responders or non-responders to chemotherapy. Western blots of soluble egg antigen (SEA) with a very sensitive chemiluminescent substrate were performed. Sera from responder children recognized a 25kDa band of SEA which diminished significantly after treatment. This was less frequent in non-responder children. When the sera of responder and non-responder children were compared before treatment, we found that the recognition of the 40 and 41 kDa proteins could be predictive of response to chemotherapy. All these antigens, used in ELISA-type techniques, might be of importance in the evaluation and follow-up of large scale schistosome control programmes. |
| Databáze: | OpenAIRE |
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