Cloning of the coat protein gene from beet necrotic yellow vein virus and its expression in sugar beet hairy roots

Autor:	W. Burgermeister, U. Ehlers, Renate Koenig, Ulrich Commandeur, R. Frank, J. Landsmann
Rok vydání:	1991
Předmět:	Furovirus biology Agrobacterium fungi General Medicine Molecular cloning biology.organism_classification Molecular biology Complementary DNA Gene expression Genetics Beet necrotic yellow vein virus Sugar beet Cauliflower mosaic virus Agronomy and Crop Science Biotechnology
Zdroj:	Theoretical and Applied Genetics. 81:777-782
ISSN:	1432-2242 0040-5752
DOI:	10.1007/bf00224989
Popis:	Expression of the beet necrotic yellow vein virus (BNYVV) coat protein (CP) gene in transgenic sugar beet hairy roots was accomplished as a step towards CP-mediated virus resistance. A cDNA for the CP gene and its 5' terminal untranslated leader sequence was prepared from BNYVV RNA, using two oligodeoxynucleotides to prime the synthesis of both strands. Second-strand synthesis and amplification of the cDNA were done by Taq DNA polymerase chain reactions. Run-off transcripts of the cloned cDNA sequence were obtained and translated in vitro, yielding immunoreactive CP. A binary vector construction containing the CP gene under the control of the 35S promoter of cauliflower mosaic virus was prepared and used for Agrobacterium rhizogenes-mediated transformation of sugar beet tissue. Stable integration and expression of the CP gene in sugar beet hairy roots was demonstrated by Southern, Northern, and Western blot analysis, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0e6ac92dbd38b8ec3ff8a67a73ca25f https://doi.org/10.1007/bf00224989 Zobrazit plný text záznamu Full text from SpringerLink