Development of Decellularized Oviductal Hydrogels as a Support for Rabbit Embryo Culture
| Autor: | Jose S. Vicente, Hannes Campo, Lucía de Miguel-Gómez, Irene Cervelló, Gianfranco Diretto, Emilio Francés-Herrero, Ximo Garcia-Dominguez, Sara López-Martínez, Francisco Marco-Jiménez, Amparo Faus |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Proteomics Fallopian tubes animal structures Embryonic Development PRODUCCION ANIMAL Extracellular matrix Embryo Culture Techniques 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hyaluronic acid Animals Metabolomics Embryo culture Hyaluronic Acid Decellularization Glycosaminoglycans 030219 obstetrics & reproductive medicine ECM Chemistry Decellularized Extracellular Matrix Embryogenesis Obstetrics and Gynecology Embryo Hydrogels Oviduct Embryonic stem cell Cell biology Culture Media Hydrogel 030104 developmental biology BIOLOGIA ANIMAL Self-healing hydrogels Female Rabbits |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| Popis: | [EN] The oviducts (fallopian tubes in mammals) function as the site of fertilization and provide necessary support for early embryonic development, mainly via embryonic exposure to the tubal microenvironment. The main objective of this study was to create an oviduct-specific extracellular matrix (oviECM) hydrogel rich in bioactive components that mimics the native environment, thus optimizing the developmental trajectories of cultured embryos. Rabbit oviducts were decellularized through SDS treatment and enzymatic digestion, and the acellular tissue was converted into oviductal pre-gel extracellular matrix (ECM) solutions. Incubation of these solutions at 37 degrees C resulted in stable hydrogels with a fibrous structure based on scanning electron microscopy. Histological staining, DNA quantification and colorimetric assays confirmed that the decellularized tissue and hydrogels contained no cellular or nuclear components but retained important components of the ECM, e.g. hyaluronic acid, glycoproteins and collagens. To evaluate the ability of oviECM hydrogels to maintain early embryonic development, two-cell rabbit embryos were cultured on oviECM-coated surfaces and compared to those cultured with standard techniques. Embryo development was similar in both conditions, with 95.9% and 98% of the embryos reaching the late morula/early blastocyst stage by 48 h under standard culture and oviECM conditions, respectively. Metabolomic analysis of culture media in the presence or absence of embryos, however, revealed that the oviECM coating may include signalling molecules and release compounds beneficial to embryo metabolism. This study was supported by Instituto de Salud Carlos III (PI17/01039-CP19/00149 [Irene Cervello]), Ministry of Economy, Industry and Competitiveness (AGL2017-85162-C2-1-R [Francisco Marco], BES-2015-072429 [Ximo Garcia]), Ministry of Science, Innovation and Universities (FPU18/06327 [Emilio Frances]) and Generalitat Valenciana (PROMETEO/2018/137 [Irene Cervello, Lucia de Miguel], ACIF/2017/118 [Sara Lopez]). The proteomics laboratory is a member of ProteoRed, PRB3, and is supported by grant PT17/0019 of the State Plan I+D+i 2013-2016, funded by Instituto de Salud Carlos III and European Regional Development Fund. |
| Databáze: | OpenAIRE |
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