Comparative cytotoxicity study of nicotine and cotinine on MRC-5 cell line
| Autor: | Daniela Luiza Baconi, Cristian Balalau, Bianca Galateanu, Ana-Maria Vlasceanu, Miriana Stan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:R5-920 business.industry MRC-5 Pharmacology Nicotine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Cell culture Medicine cytotoxicity MTT assay cotinine Cotinine business Cytotoxicity lcsh:Medicine (General) MTT-assay medicine.drug nicotine |
| Zdroj: | Journal of Mind and Medical Sciences, Vol 5, Iss 1, Pp 117-122 (2018) |
| ISSN: | 2392-7674 |
| Popis: | Nicotine has several health hazards regarding carcinogenic potential. It also imparts increased risk for respiratory, cardiovascular, and gastrointestinal disorders. Several mechanisms have been proposed for the carcinogenic potential, including effects on cell proliferation, inducing oxidative stress, DNA mutation, or inhibition of apoptosis. The cotinine metabolite is generally thought to have effects similar to nicotine in some experimental systems. The purpose of this study was to assess the nicotine and cotinine cytotoxicity on MRC-5 lung fibroblasts. The pulmonary fibroblasts were treated with various concentrations of nicotine or cotinine (in the range 1 µM – 2 mM) for 24 or 48 h and analyzed for cell viability by MTT test. The results indicated that high nicotine concentrations (2 mM) induced marked cell death (about 50%) in MRC-5 cell line. Cotinine showed lower toxicity than nicotine on the MRC-5 cells. In contrast to nicotine treatment, cells treated with cotinine continued to proliferate after the 48h incubation period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|