A search forPneumocystis carinii in post-mortem lungs by DNA amplification
| Autor: | Sarah E. Peters, Kathryn Sinclair, Ann F. Wakefield, Peter R. Millard, J. M. Hopkin |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
Oligonucleotide hybridization Adolescent Biology Polymerase Chain Reaction Pathology and Forensic Medicine chemistry.chemical_compound Ethidium Gene duplication medicine Humans Lung Aged Electrophoresis Agar Gel Staining and Labeling Pneumocystis Medical screening Respiratory disease Middle Aged Dna amplification medicine.disease Virology respiratory tract diseases medicine.anatomical_structure chemistry Pneumocystis carinii Oligonucleotide Probes DNA |
| Zdroj: | The Journal of Pathology. 166:195-198 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/path.1711660217 |
| Popis: | DNA amplification of specific sequences and subsequent oligonucleotide hybridization were used to search for Pneumocystis carinii in post-mortem lung samplings from non-immunosuppressed individuals ranging from 15 to 70 years of age. No P. carinii-specific DNA was detected in 45 DNA amplification reactions from 15 lungs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text