ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1
| Autor: | Boris Simonetti, James L. Daly, Lorena Simón-Gracia, Katja Klein, Saroja Weeratunga, Carlos Antón-Plágaro, Allan Tobi, Lorna Hodgson, Phil Lewis, Kate J. Heesom, Deborah K. Shoemark, Andrew D. Davidson, Brett M. Collins, Tambet Teesalu, Yohei Yamauchi, Peter J. Cullen |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences (PNAS) Proceedings of the National Academy of Sciences of the United States of America, 119 (25) bioRxiv Simonetti, B, Daly, J, Klein, K, Anton Plagaro, C, Hodgson, L R, Heesom, K J, Shoemark, D K, Davidson, A D, Yamauchi, Y, Cullen, P J & et, A 2022, ' ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1 ', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 25, 2201980119 . https://doi.org/10.1073/pnas.2201980119 |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.3929/ethz-b-000588193 |
| Popis: | Endosomal sorting maintains cellular homeostasis by recycling transmembrane proteins and associated proteins and lipids (termed “cargoes”) from the endosomal network to multiple subcellular destinations, including retrograde traffic to the trans-Golgi network (TGN). Viral and bacterial pathogens subvert retrograde trafficking machinery to facilitate infectivity. Here, we develop a proteomic screen to identify retrograde cargo proteins of the endosomal SNX-BAR sorting complex promoting exit 1 (ESCPE-1). Using this methodology, we identify Neuropilin-1 (NRP1), a recently characterized host factor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, as a cargo directly bound and trafficked by ESCPE-1. ESCPE-1 mediates retrograde trafficking of engineered nanoparticles functionalized with the NRP1-interacting peptide of the SARS-CoV-2 spike (S) protein. CRISPR-Cas9 deletion of ESCPE-1 subunits reduces SARS-CoV-2 infection levels in cell culture. ESCPE-1 sorting of NRP1 may therefore play a role in the intracellular membrane trafficking of NRP1-interacting viruses such as SARS-CoV-2. Proceedings of the National Academy of Sciences of the United States of America, 119 (25) ISSN:0027-8424 ISSN:1091-6490 |
| Databáze: | OpenAIRE |
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