Plants with genetically encoded autoluminescence

Autor:	Alexander S. Mishin, Vadim V. Negrebetsky, Tatiana A. Karataeva, Ekaterina S. Shakhova, Dmitry Shlykov, Alexander Pushin, Tatiana V. Chepurnyh, Nadezhda M. Markina, Liliia I. Fakhranurova, Kseniia A. Palkina, Sergey A. Dolgushin, Aleksandra S. Tsarkova, Pavel V. Shalaev, Tatiana Mitiouchkina, Sergey Dolgov, Yaroslav V. Golubev, Sergey M. Deyev, Elena B. Guglya, Louisa Gonzalez Somermeyer, Fyodor A. Kondrashov, Andrey I. Bubyrev, Ilia V. Yampolsky, Olesya A. Melnik, Karen S. Sarkisyan, Victoria O. Shipunova, Vladimir V. Choob, Sofia V. Chekova
Rok vydání:	2020
Předmět:	Biomedical Engineering Bioengineering Firefly Luciferin Biology Applied Microbiology and Biotechnology Article 03 medical and health sciences chemistry.chemical_compound Caffeic Acids 0302 clinical medicine Tobacco Gene cluster Caffeic acid Bioluminescence Plastid 030304 developmental biology 0303 health sciences fungi Fungi food and beverages Plants Genetically Modified Luciferin Biochemistry chemistry Molecular Medicine Naked eye 030217 neurology & neurosurgery Biotechnology
Zdroj:	Nature Biotechnology Nature biotechnology
ISSN:	1546-1696 1087-0156
DOI:	10.1038/s41587-020-0500-9
Popis:	Autoluminescent plants that express a bacterial bioluminescence gene cluster1 have not been widely adopted due to requisite expression in plastids and low light output. Alternatively, we have engineered tobacco lines expressing a fungal bioluminescent system2, which converts caffeic acid present in all plants into luciferin, and report self-sustained luminescence easily visible to the naked eye. Our findings might underpin development of a suite of imaging tools for plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0df9fcc59fcde31ad10943989f9a02b https://doi.org/10.1038/s41587-020-0500-9 Zobrazit plný text záznamu Full text from SpringerLink