Intramuscular injection of 125I-botulinum neurotoxin-complex versus 125I-botulinum-free neurotoxin: time course of tissue distribution
| Autor: | Leslie F Chasseaud, Diane D.-S. Tang-Liu, J. Oliver Dolly, Colin Webber, Tara L Houchen, K. Roger Aoki, Anton de Paiva |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Botulinum Toxins Biology Pharmacology Toxicology medicine.disease_cause Injections Intramuscular Diffusion Iodine Radioisotopes Rats Sprague-Dawley Gastrocnemius muscle Pharmacokinetics medicine Neurotoxin Animals Tissue Distribution Botulinum Toxins Type A Muscle Skeletal chemistry.chemical_classification Toxin Thyroid Eyelids Rats Molecular Weight Kinetics medicine.anatomical_structure Enzyme chemistry Toxicity Rabbits Intramuscular injection |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 42(5) |
| ISSN: | 0041-0101 |
| Popis: | The diffusion from the site of intramuscular injection of 900 kDa botulinum neurotoxin-hemagglutinin complex (BoNT/A-complex) and 150 kDa free-botulinum neurotoxin (free-BoNT/A) was compared. Radioiodinated compounds were injected into the gastrocnemius muscle of rats (70Units (U) 125I-BoNT/A-complex, 67 or 344 U free-125I-BoNT/A, or free-125I-iodide) and the eyelids of rabbits (24 U 125I-BoNT/A-complex or 108 U free-125I-BoNT/A), and measured in various tissues at different time points. There were no detectable systemic effects or generalized botulinum neurotoxin toxicity in either rats or rabbits, indicating that most of the toxin, whether as 125I-BoNT/A-complex or free-125I-BoNT/A, remained at the injection site. In rats, 125I-BoNT/A-complex and free-125I-BoNT/A diffused in a pattern that was grossly similar. Almost no radioactivity was recovered from the brain. Radioactivity recovered from distant tissues (thyroid, skin, and contralateral muscle) was primarily attributable to either low molecular weight 125I-containing peptides or 125I-iodide. After injection into rabbit eyelids, neither 125I-BoNT/A-complex nor free-125I-BoNT/A spread to distant structures, including the eye. The results indicate that most of the neurotoxin does not diffuse from the injection site, whether in free or complexed form, and this may reduce the potential for systemic effects. |
| Databáze: | OpenAIRE |
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