Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties
| Autor: | Tamara Tilburgs, Errol R. Norwitz, Jack L. Strominger, Sarika Kshirsagar, Vidya Iyer, Lily Xu, Hannah Ananda Bougleux Gomes, Henrieta Papuchova, Qin Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Spiral artery Placenta Biology Preeclampsia Cell Line 03 medical and health sciences 0302 clinical medicine Immune system Pre-Eclampsia Cell Movement Pregnancy HLA-G medicine Humans HLA-G Antigens Fetus 030219 obstetrics & reproductive medicine Multidisciplinary Trophoblast Gene Expression Regulation Developmental Biological Sciences medicine.disease Immunity Innate Placentation Trophoblasts Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure Immunology Maternal-Fetal Relations Female |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | During pregnancy, invading HLA-G+ extravillous trophoblasts (EVT) play a key role in placental development, uterine spiral artery remodeling, and prevention of detrimental maternal immune responses to placental and fetal antigens. Failures of these processes are suggested to play a role in the development of pregnancy complications, but very little is known about the underlying mechanisms. Here we present validated methods to purify and culture primary HLA-G+ EVT from the placental disk and chorionic membrane from healthy term pregnancy. Characterization of HLA-G+ EVT from term pregnancy compared to first trimester revealed their unique phenotypes, gene expression profiles, and differing capacities to increase regulatory T cells (Treg) during coculture assays, features that cannot be captured by using surrogate cell lines or animal models. Furthermore, clinical variables including gestational age and fetal sex significantly influenced EVT biology and function. These methods and approaches form a solid basis for further investigation of the role of HLA-G+ EVT in the development of detrimental placental inflammatory responses associated with pregnancy complications, including spontaneous preterm delivery and preeclampsia. |
| Databáze: | OpenAIRE |
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