Molecular characterisation and disease severity of leptospirosis in Sri Lanka
| Autor: | Nilantha Ranasinghe, Manjula Weerasekera, Chinthika Gunasekara, Kanchana Bandara, Chamil Marasinghe, Neluka Fernando |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Serotype Adult Male lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 lcsh:QR1-502 Polymerase Chain Reaction Severity of Illness Index Chromatography Affinity lcsh:Microbiology Species Specificity Leptospira Direct agglutination test Agglutination Tests medicine Animals Humans Leptospirosis Prospective Studies Subclinical infection Sri Lanka Polymorphism Genetic biology Outbreak molecular characterisation Articles Sequence Analysis DNA biology.organism_classification medicine.disease Virology Leptospira kirschneri Female Leptospira interrogans |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 110, Iss 4, Pp 485-491 (2015) Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 110, Issue: 4, Pages: 485-491, Published: 09 JUN 2015 |
| ISSN: | 1678-8060 |
| Popis: | Leptospirosis is a re-emerging zoonotic disease all over the world, important in tropical and subtropical areas. A majority of leptospirosis infected patients present as subclinical or mild disease while 5-10% may develop severe infection requiring hospitalisation and critical care. It is possible that several factors, such as the infecting serovar, level of leptospiraemia, host genetic factors and host immune response, may be important in predisposition towards severe disease. Different Leptospira strains circulate in different geographical regions contributing to variable disease severity. Therefore, it is important to investigate the circulating strains at geographical locations during each outbreak for epidemiological studies and to support the clinical management of the patients. In this study immunochromatography, microscopic agglutination test and polymerase chain reaction were used to diagnose leptospirosis. Further restriction fragment length polymorphism and DNA sequencing methods were used to identify the circulating strains in two selected geographical regions of Sri Lanka. Leptospira interrogans, Leptospira borgpetersenii and Leptospira kirschneri strains were identified to be circulating in western and southern provinces. L. interrogans was the predominant species circulating in western and southern provinces in 2013 and its presence was mainly associated with renal failure. |
| Databáze: | OpenAIRE |
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