Accurate correction method and algorithm of fluorescence secondary inner filter effect (sIEF) in fluorescence quantitative analysis

Autor:	Wanxiang Li, Yuchao Fu, Tianyuan Liu, Haochen Li, Meizhen Huang
Rok vydání:	2022
Předmět:	Instrumentation Spectroscopy Atomic and Molecular Physics and Optics Analytical Chemistry
Zdroj:	Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 288
ISSN:	1873-3557
Popis:	Fluorescence spectroscopy is a reliable and widely used analytical method. The fluorescence inner filter effect (IFE) is one of the main obstacles in the application of fluorescence spectroscopy and an error source in fluorescence analysis, resulting in the fluorescence spectrum distortion, the spectral shape distortion, and the nonlinearity between fluorescence intensity and fluorophore concentration. An optimized parameter reflecting the self-absorption effect - the fluorescence attenuation absorption index of secondary inner filter effect (sIFE) n
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0d4eb95a6b62509cc2f537df1dce480 https://pubmed.ncbi.nlm.nih.gov/36473295 Zobrazit plný text záznamu Full Text from ScienceDirect