Kinetics of thrombomodulin release and endothelial cell injury by neutrophil-derived proteases and oxygen radicals
| Autor: | Peter R. Galle, M. W. J. Boehme, Wolfgang Stremmel |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Proteases
Endothelium Cell Survival Neutrophils Thrombomodulin Immunology Cell Culture Techniques Apoptosis Biology Cathepsin G chemistry.chemical_compound Endopeptidases Cell Adhesion medicine Humans Immunology and Allergy Viability assay Cathepsin Hydrogen Peroxide Original Articles Molecular biology Endothelial stem cell medicine.anatomical_structure Microscopy Fluorescence chemistry Endothelium Vascular Reactive Oxygen Species |
| Zdroj: | Immunology. 107:340-349 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | Thrombomodulin is a transmembranous glycoprotein of endothelial cells. In vitro it is a marker of endothelial cell injury. In vivo the levels of serum thrombomodulin are regarded as a parameter of activity in vasculitides. The latter are pathophysiologically determined by neutrophil-derived inflammation and endothelial cell injury caused by secretion of proteases and hydrogen peroxide. It was the objective of this study to determine whether thrombomodulin is only a late marker of advanced endothelial cell injury or whether it indicates also earlier stages of cell alterations. Over 24 hr endothelial cell cultures were incubated with hydrogen peroxide or the neutrophil proteases proteinase-3, elastase and cathepsin G. The time-dependent increase of thrombomodulin in the supernatant was determined by enzyme-linked immunosorbent assay and immunoblot. In addition the viability (eosin, tetrazolium dye assay), detachment (crystal-violet assay), and apoptosis (4′,6-diamine-2′-phenylindole-dihydrochloride assay) of the respective endothelial cells were determined for adherent and non-adherent cells. A rapid thrombomodulin increase was found under all experimental conditions. The additional immunoblotting analysis showed the pattern of proteolytic cleavage caused by the protease reactivity. In case of hydrogen peroxide the thrombomodulin increase was closely correlated with the loss of cell viability and lysis. The incubation of endothelial cells with the different proteases resulted in a time-dependent detachment of primarily viable cells. In addition to cell necrosis apoptotic cell death was found in the subgroup of detached endothelial cells after prolonged incubation over 24 hr with proteinase-3 (23%), elastase (31%), and cathepsin G (19%). In contrast, still adhering cells did not show any signs of necrosis or apoptosis. In summary these studies confirm in vitro that soluble thrombomodulin is not only a parameter of advanced endothelial cell destruction itself but also in addition an early marker of initial endothelial cell membrane changes induced by neutrophil derived proteases and oxygen radicals. |
| Databáze: | OpenAIRE |
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