Expression of MicroRNA-146a and MicroRNA-155 in Placental Villi in Early- and Late-Onset Preeclampsia
| Autor: | M N Nagovitsyna, N V Nizyaeva, N. E. Kan, G V Kulikova, K N Prozorovskaya, Shchegolev Ai, Gennady T. Sukhikh |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Time Factors Pregnancy Trimester Third Gestational Age In situ hybridization Biology General Biochemistry Genetics and Molecular Biology Preeclampsia Andrology 03 medical and health sciences Fetus 0302 clinical medicine Syncytiotrophoblast Pre-Eclampsia Pregnancy Placenta medicine Humans In Situ Hybridization reproductive and urinary physiology Trophoblast Gestational age General Medicine medicine.disease female genital diseases and pregnancy complications MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Case-Control Studies embryonic structures Gestation Female Chorionic Villi 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 163:394-399 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | We studied the expression of microRNA-146a and microRNA-155 in placental villi from 18 women (26-39 weeks of gestation) of reproductive age with early- or late-onset preeclampsia. The reference group consisted of women with physiological pregnancy and full-term gestation and with preterm birth after caesarian section on gestation week 26-31. MicroRNA-146a and microRNA-155 were detected by in situ hybridization with digoxigenin on paraffin sections. It was found that the expression of microRNA-146a in both syncytiotrophoblast of the intermediate villi and syncytial knots was lower at late-onset preeclampsia than at physiologic pregnancy of full-term period (p=0.037 and p=0.001 respectively). The expression of microRNA-155 in syncytiotrophoblast of intermediate placental villi in early-onset preeclampsia was higher than in group with preterm delivery (p=0.003). However, in syncytiotrophoblast of intermediate villi and in syncytial knots, the expression of microRNA-155 was lower at late-onset preeclampsia in comparison with full-term physiological pregnancy (p=0.005). In addition, the expression of microRNA-146a and microRNA-155 did not increase in the later terms in preeclampsia, while in the reference groups demonstrating gradual increase in the expression of these markers with increasing gestational age. Expression microRNA-146a and microRNA-155 little differed in early- and late-onset preeclampsia. These findings suggest that different variants of preeclampsia are probably characterized by common pathogenetic pathways. Damaged trophoblast cannot maintain of microRNAs synthesis at the required level, which determines the formation of a vicious circle in preeclampsia and further progression of the disease. |
| Databáze: | OpenAIRE |
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