Purification, characterization and antioxidant activities of a polysaccharide from the roots of Lilium davidii var. unicolor Cotton
| Autor: | Ruiming Zhao, Hui Jin, Xiaoyan Yang, Xiuzhuang Li, Haiyan Cui, Heping Hui, Aiyi Xin, Bo Qin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chemical Phenomena
DPPH Mannose Glucomannan 02 engineering and technology Polysaccharide Methylation Plant Roots Biochemistry Antioxidants Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Polysaccharides Structural Biology Lilium davidii Molecular Biology Ethanol precipitation 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography biology General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Hot water extraction chemistry Thermogravimetry Hydroxyl radical Lilium 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 135:1208-1216 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.06.030 |
| Popis: | A polysaccharide (LPR) was separated from the roots of Lilium davidii var. unicolor Cotton with hot water extraction, ethanol precipitation, and purification by anion-exchange and gel-permeation chromatography. LPR was characterized. The weight-average molecular weight (MW) of LPR was 5.12 × 104 g/moL. Glucose and mannose comprised LPR with a molar ratio of 2.9:3.3. IR, NMR and methylation analysis showed that LPR was a natural O-acetyl glucomannan, the backbone mainly contained β-(1 → 4)-linked d -glucopyranosyl and β-(1 → 4)-linked D-mannopyanosyl, and the branches probably linked at O-2 and/or O-3 of the mannosyl and glucosyl residues. The acetyl groups mainly attached at O-2 or O-3 of mannosyl residues. X-ray diffractometric (XRD) analysis and scanning electron microscopy (SEM) analysis revealed that LPR was a semi-crystalline substance with porous lamellar structure. Bioassays in vitro indicated that LPR had distinct scavenging activities on hydroxyl radical and DPPH radical. These findings provided a reference for functional underutilization roots of L. davidii as natural antioxidant in food and pharmaceutical industry. |
| Databáze: | OpenAIRE |
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