Arthritis in systemic lupus erythematosus is characterized by local IL-17A and IL-6 expression in synovial fluid

Autor: Vivianne Malmström, Francesca Faustini, Iva Gunnarsson, Sara Turcinov, Natalie Sippl, Johan Rönnelid, Karine Chemin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
CD4-Positive T-Lymphocytes
Male
0301 basic medicine
T cell
Immunology
T cells
Arthritis
CD8-Positive T-Lymphocytes
Interferon-gamma
Editors' Choice
03 medical and health sciences
0302 clinical medicine
synovial fluid
systemic lupus erythematosus
immune system diseases
medicine
Humans
Lupus Erythematosus
Systemic
Immunology and Allergy
Synovial fluid
Interleukin 6
skin and connective tissue diseases
B cell
Rheumatology and Autoimmunity
B-Lymphocytes
Reumatologi och inflammation
Systemic lupus erythematosus
biology
Interleukin-6
business.industry
Interleukin-17
Immunology in the medical area
Dendritic cell
Middle Aged
medicine.disease
cytokines
030104 developmental biology
medicine.anatomical_structure
arthritis
Rheumatoid arthritis
Immunologi inom det medicinska området
biology.protein
Th17 Cells
Female
Original Article
business
030215 immunology
Zdroj: Clinical and Experimental Immunology
Popis: Summary Arthritis is a common clinical feature of systemic lupus erythematosus (SLE) and is usually non‐erosive, as opposed to rheumatoid arthritis (RA). While RA synovial pathology has been extensively studied, little is known about the pathophysiology of lupus arthritis. Here, we aimed to explore the cytokine and cellular compartments in synovial fluids of SLE patients with arthritic manifestations. Acellular synovial fluid and paired serum samples from SLE patients (n = 17) were analyzed with cytokine bead array for T helper‐associated cytokines. From two SLE patients, synovial fluid mononuclear cells (SFMC) could also be captured and were analyzed by multiparameter flow cytometry to dissect T cell, B cell, monocyte and dendritic cell phenotypes. SLE‐derived SFMC were further stimulated in vitro to measure their capacity for producing interferon (IFN)‐γ and interleukin (IL)‐17A. All patients fulfilled the ACR 1982 classification criteria for SLE. Clinical records were reviewed to exclude the presence of co‐morbidities such as osteoarthritis or overlap with RA. IL‐17A and IL‐6 levels were high in SLE synovial fluid. A clear subset of the synovial CD4+ T cells expressed CCR6+, a marker associated with T helper type 17 (Th17) cells. IL‐17A‐production was validated among CD4+CCR6+ T cells following in‐vitro stimulation. Furthermore, a strong IFN‐γ production was observed in both CD4+ and CD8+ cells. Our study shows high IL‐17A and IL‐6 levels in synovial fluids of patients with lupus arthritis. The Th17 pathway has been implicated in several aspects of SLE disease pathogenesis and our data also point to Th17 involvement for lupus arthritis.
IL‐17A and IL‐6 are elevated in synovial fluid of SLE arthritis patients. The Th17 pathway have been implicated in several aspects of SLE disease pathogenesis and our data points to Th17 involvement also for lupus arthritis.
Databáze: OpenAIRE
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