Marker genes identify three somatic cell types in the fetal mouse ovary
| Autor: | James S. Palmer, Marie-Christine Chaboissier, Pascal Bernard, Robert G. Ramsay, Huijun Chen, Dagmar Wilhelm, Patrick S. Western, Anne-Amandine Chassot, Raphael H. Rastetter |
|---|---|
| Přispěvatelé: | Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Forkhead Box Protein L2
Somatic cell Fluorescent Antibody Technique MESH: Receptors G-Protein-Coupled MESH: Genetic Markers Receptors G-Protein-Coupled COUP Transcription Factor II Mice WNT4 MESH: Gene Expression Regulation Developmental MESH: Animals MESH: Fluorescent Antibody Technique [SDV.BDD]Life Sciences [q-bio]/Development Biology In Situ Hybridization education.field_of_study MESH: Real-Time Polymerase Chain Reaction Gene Expression Regulation Developmental Forkhead Transcription Factors Cell biology Foxl2 medicine.anatomical_structure MESH: COUP Transcription Factor II Female Stem cell Germ cell Adult stem cell Genetic Markers MESH: Ovary medicine.medical_specialty Population Ovary Biology Real-Time Polymerase Chain Reaction Rspo1 Lgr5 Fetus MESH: In Situ Hybridization MESH: Mice Inbred C57BL MESH: Forkhead Transcription Factors Internal medicine medicine Animals Cell Lineage education Nr2f2 Molecular Biology MESH: Mice MESH: Fetus Cell Biology MESH: Cell Lineage Mice Inbred C57BL Endocrinology Wnt4 Developmental biology MESH: Female Developmental Biology |
| Zdroj: | Developmental Biology Developmental Biology, Elsevier, 2014, 394 (2), pp.242-52 |
| ISSN: | 0012-1606 1095-564X |
| Popis: | International audience; The two main functions of the ovary are the production of oocytes, which allows the continuation of the species, and secretion of female sex hormones, which control many aspects of female development and physiology. Normal development of the ovaries during embryogenesis is critical for their function and the health of the individual in later life. Although the adult ovary has been investigated in great detail, we are only starting to understand the cellular and molecular biology of early ovarian development. Here we show that the adult stem cell marker Lgr5 is expressed in the cortical region of the fetal ovary and this expression is mutually exclusive to FOXL2. Strikingly, a third somatic cell population can be identified, marked by the expression of NR2F2, which is expressed in LGR5- and FOXL2 double-negative ovarian somatic cells. Together, these three marker genes label distinct ovarian somatic cell types. Using lineage tracing in mice, we show that Lgr5-positive cells give rise to adult cortical granulosa cells, which form the follicles of the definitive reserve. Moreover, LGR5 is required for correct timing of germ cell differentiation as evidenced by a delay of entry into meiosis in Lgr5 loss-of-function mutants, demonstrating a key role for LGR5 in the differentiation of pre-granulosa cells, which ensure the differentiation of oogonia, the formation of the definitive follicle reserve, and long-term female fertility. |
| Databáze: | OpenAIRE |
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