Eukaryotic Initiation Factors (eIF) 2α and 4E Expression, Localization, and Phosphorylation in Brain Tumors
| Autor: | Silvia Sacristán, M Val T Lobo, Mercedes García-Villanueva, M. Isabel Pérez-Morgado, M. Elena Martín, Sonia Tejada, Matilde Salinas |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Histology Eukaryotic Initiation Factor-2 Biology Article Young Adult Cyclin D1 Eukaryotic translation Eukaryotic initiation factor Biomarkers Tumor Humans Initiation factor Oligodendroglial Tumor Phosphorylation Aged Cell Proliferation Aged 80 and over Brain Neoplasms Middle Aged Cell cycle Subcellular localization Cell biology Eukaryotic Initiation Factor-4E Ki-67 Antigen Cancer cell Cancer research Female Tumor Suppressor Protein p53 Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 57:503-512 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | Increased protein synthesis is regulated, in part, by two eukaryotic translation initiation factors (eIFs): eIF4E and eIF2α. One or both of these factors are often overexpressed in several types of cancer cells; however, no data are available at present regarding eIF4E and eIF2α levels in brain tumors. In this study, we analyzed the expression, subcellular localization and phosphorylation states of eIF4E and eIF2α in 64 brain tumors (26 meningiomas, 16 oligodendroglial tumors, and 22 astrocytomas) and investigated the correlation with the expression of MIB-1, p53, and cyclin D1 proteins as well. There are significant differences in the phosphorylated eIF4E levels between the tumors studied, being the highest in meningiomas and the lowest in the oligodendroglial tumors. Relative to subcellular localization, eIF4E is frequently found in the nucleus of the oligodendroglial tumors and rarely in the same compartment of the meningiomas, whereas eIF2α showed an inverse pattern. Finally, cyclin D1 levels directly correlate with the phosphorylation status of both factors. The different expression, phosphorylation, or/and subcellular distribution of eIF2α and eIF4E within the brain types of tumors studied could indicate that different pathways are activated for promoting cell cycle proliferation, for instance, leading to increased cyclin D1 expression. (J Histochem Cytochem 57:503–512, 2009) |
| Databáze: | OpenAIRE |
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