Analysis of several PLA2 mRNA in human meningiomas
| Autor: | Yves Denizot, François Labrousse, Karine Durand, François Caire, J.-J. Moreau, Nicolas Weinbreck, Rafael De Armas, Sandrine Robert |
|---|---|
| Přispěvatelé: | Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations (PMRIL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS), Service d'Hématologie biologique [CHU Limoges], CHU Limoges, Archéologies et Sciences de l'Antiquité (ArScAn), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Service de Neurochirurgie [CHU Limoges], Université de Limoges (UNILIM), Service de Neurologie [CHU Limoges], Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Institut national de recherches archéologiques préventives (Inrap)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Necrosis MESH: Meningioma chemistry.chemical_compound 0302 clinical medicine Edema MESH: Reverse Transcriptase Polymerase Chain Reaction Meningeal Neoplasms RNA Neoplasm MESH: Phospholipases A2 0303 health sciences MESH: Middle Aged Reverse Transcriptase Polymerase Chain Reaction Middle Aged respiratory system 030220 oncology & carcinogenesis [SDV.IMM]Life Sciences [q-bio]/Immunology Female lipids (amino acids peptides and proteins) Inflammation Mediators medicine.symptom Meningioma Intracellular Research Article lcsh:RB1-214 Article Subject Immunology MESH: Inflammation Mediators Phospholipid macromolecular substances Biology 03 medical and health sciences stomatognathic system medicine otorhinolaryngologic diseases lcsh:Pathology Humans RNA Messenger Gene 030304 developmental biology MESH: RNA Messenger Messenger RNA Phospholipase A MESH: Humans Cell Biology equipment and supplies MESH: Meningeal Neoplasms MESH: RNA Neoplasm Molecular biology MESH: Male Phospholipases A2 Eicosanoid chemistry MESH: Female |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Hindawi Publishing Corporation, 2009, 2009, pp.689430. ⟨10.1155/2009/689430⟩ Mediators of Inflammation, 2009, 2009, pp.689430. ⟨10.1155/2009/689430⟩ Mediators of Inflammation, Vol 2009 (2009) |
| ISSN: | 0962-9351 1466-1861 |
| DOI: | 10.1155/2009/689430⟩ |
| Popis: | In view of the important oncogenic action of phospholipaseA2(PLA2)we investigatedPLA2transcripts in human meningiomas. Real-time PCR was used to investigatePLA2transcripts in 26 human meningioma tumors. Results indicated that threeCa2+-dependent high molecular weightPLA2(PLA2-IVA,PLA2-IVB,PLA2-IVC), oneCa2+-independent high molecular weightPLA2(PLA2-VI) and five low molecular weight secreted forms ofPLA2(PLA2-IB,PLA2-IIA,PLA2-III,PLA2-V, andPLA2-XII) are expressed withPLA2-IVA,PLA2-IVB,PLA2-VI, andPLA2-XIIA as the major expressed forms.PLA2-IIE,PLA2-IIF,PLA2-IVD, andPLA2-XIIB are not detected. Plasma (PLA2-VIIA) and intracellular (PLA2-VIIB) platelet-activating factor acetylhydrolase transcripts are expressed in human meningiomas. However no difference was found forPLA2transcript amounts in relation to the tumor grade, the subtype of meningiomas, the presence of inflammatory infiltrated cells, of an associated edema, mitosis, brain invasion, vascularisation or necrosis. In conclusion numerous genes encoding multiples forms ofPLA2are expressed in meningiomas where they might act on the phospholipid remodeling and on the local eicosanoid and/or cytokine networks. |
| Databáze: | OpenAIRE |
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