Aligned collagen scaffold combination with human spinal cord-derived neural stem cells to improve spinal cord injury repair
| Autor: | Jianwu Dai, Weiwei Xue, Yunlong Zou, Rui Gu, Bing Chen, Yannan Zhao, Zhifeng Xiao, Zheng Sun, Ya Shi, Bai Xu, Yongheng Fan, He Shen, Dezun Ma |
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| Rok vydání: | 2020 |
| Předmět: |
Spinal Cord Regeneration
Biomedical Engineering 02 engineering and technology Glial scar 03 medical and health sciences Neural Stem Cells medicine Animals Humans General Materials Science Progenitor cell Spinal cord injury Spinal Cord Injuries 030304 developmental biology 0303 health sciences business.industry Cell Differentiation Recovery of Function 021001 nanoscience & nanotechnology Spinal cord medicine.disease Neural stem cell Rats Cell biology Transplantation medicine.anatomical_structure Spinal Cord Collagen Stem cell 0210 nano-technology business |
| Zdroj: | Biomaterials Science. 8:5145-5156 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/d0bm00431f |
| Popis: | Neural stem/progenitor cell (NSPC)-based spinal cord injury (SCI) therapy is expected to bridge the lesion site by transplanting exogenous NSPCs for replacement of lost cells. The transplanted NSPCs produce a microenvironment conducive to neuronal regeneration, and ultimately, functional recovery. Although both human fetal brain- and spinal cord- derived NSPCs (hbNSPCs and hscNSPCs, respectively) have been used for SCI repair, it remains unclear whether hscNSPCs are a more appropriate stem cell source for transplantation than hbNSPCs. Therefore, in this study, we transplanted hbNSPCs or hscNSPCs into rats with complete transection SCI to monitor their differences in SCI treatment. An aligned collagen sponge scaffold (ACSS) was used here for cell retention. Aligned biomaterial scaffolds provide a support platform and favorable morphology for cell growth and differentiation, and guide axial axonal extension. The ACSS fabricated by our group has been previously reported to improve spinal cord repair by promoting neuronal regeneration and remyelination. Compared with the hbNSPC-ACSS, the hscNSPC-ACSS effectively promoted long-term cell survival and neuronal differentiation and improved the SCI microenvironment by reducing inflammation and glial scar formation. Furthermore, the transplanted hscNSPC-ACSS improved recovery of locomotor functions. Therefore, hscNSPCs appear to be a superior cell source to hbNSPCs for SCI cell therapy with greater potential clinical applications. |
| Databáze: | OpenAIRE |
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