Microtubule-driven nuclear rotations promote meiotic chromosome dynamics
| Autor: | Nicolas Christophorou, Thomas Rubin, Tristan Piolot, Jean-René Huynh, Isabelle Bonnet, Marion Arnaud |
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| Přispěvatelé: | Génétique et Biologie du Développement, Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Université Grenoble Alpes - UFR Médecine (UGA UFRM), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes (UGA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
germline
Animals Genetically Modified pairing 0302 clinical medicine Mud Drosophila Proteins meiosis SUN and KASH domain proteins 0303 health sciences Microscopy Confocal Synaptonemal Complex oogenesis Dynein Cell biology Synaptonemal complex Drosophila melanogaster medicine.anatomical_structure Female RNA Interference Drosophila Protein Binding Rotation Centromere Nerve Tissue Proteins [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Time-Lapse Imaging microtubules 03 medical and health sciences Meiosis Microtubule medicine Animals Mitosis 030304 developmental biology Cell Nucleus Centrosome Ovary Dyneins Membrane Proteins Membrane Transport Proteins Cell Biology Chromosome Pairing Kinetics Luminescent Proteins Cell nucleus 030217 neurology & neurosurgery |
| Zdroj: | Nature Cell Biology Nature Cell Biology, Nature Publishing Group, 2015, 17 (11), pp.1388-1400. ⟨10.1038/ncb3249⟩ Nature Cell Biology, 2015, 17 (11), pp.1388-1400. ⟨10.1038/ncb3249⟩ |
| ISSN: | 1465-7392 1476-4679 |
| Popis: | International audience; At the onset of meiosis, each chromosome needs to find its homologue and pair to ensure proper segregation. In Drosophila, pairing occurs during the mitotic cycles preceding meiosis. Here we show that germ cell nuclei undergo marked movements during this developmental window. We demonstrate that microtubules and Dynein are driving nuclear rotations and are required for centromere pairing and clustering. We further found that Klaroid (SUN) and Klarsicht (KASH) co-localize with centromeres at the nuclear envelope and are required for proper chromosome motions and pairing. We identified Mud (NuMA in vertebrates) as co-localizing with centromeres, Klarsicht and Klaroid. Mud is also required to maintain the integrity of the nuclear envelope and for the correct assembly of the synaptonemal complex. Our findings reveal a mechanism for chromosome pairing in Drosophila, and indicate that microtubules, centrosomes and associated proteins play a crucial role in the dynamic organization of chromosomes inside the nucleus. |
| Databáze: | OpenAIRE |
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