Combination Therapy of Wuweizi (Schisandrae Chinensis Fructus) and Dexamethasone Alleviated Dexamethasone-Induced Glucocorticoid Osteoporosis in Rats with Idiopathic Pulmonary Fibrosis

Autor: Hua-Qiang Zhai, Guo-Xiu Liu, Zinan Qin, Nan Zhang, Min Gu, Jiao Liu, Yongpeng Han, Zhihong Ji, Xiaojuan Zhang, Keao Li, Si-Yu Li
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
medicine.medical_treatment
Osteoporosis
Dexamethasone
Bone remodeling
0302 clinical medicine
Bone Marrow
Pulmonary fibrosis
Basic Helix-Loop-Helix Transcription Factors
Lung
Schisandra
Platelet-Derived Growth Factor
General Medicine
Endostatins
030220 oncology & carcinogenesis
Cancellous Bone
Medicine
Endostatin
Glucocorticoid
Research Article
medicine.drug
medicine.medical_specialty
Article Subject
Combination therapy
Bone and Bones
General Biochemistry
Genetics and Molecular Biology
Bleomycin
03 medical and health sciences
Internal medicine
medicine
Animals
Nerve Growth Factors
Rats
Wistar
Eye Proteins
Glucocorticoids
Serpins
General Immunology and Microbiology
Plant Extracts
Tartrate-Resistant Acid Phosphatase
business.industry
Growth factor
medicine.disease
Idiopathic Pulmonary Fibrosis
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
business
Drugs
Chinese Herbal
Zdroj: BioMed Research International
BioMed Research International, Vol 2020 (2020)
ISSN: 2314-6141
2314-6133
Popis: Objective To investigate the therapeutic effect of combined application of Wuweizi (Schisandrae Chinensis Fructus) and dexamethasone in rats with idiopathic pulmonary fibrosis (IPF) and the possible protective effect of Wuweizi against dexamethasone-induced glucocorticoid osteoporosis (GIOP). Methods There were five groups in this study, including the sham operation group, model group, Wuweizi group, dexamethasone group, and the combination group. A rat IPF model was made by the endotracheal injection of bleomycin. After modeling, rats were given drug interventions for 7 and 28 days. Rats were sacrificed for pathological morphology examination of the bone and lung and quantitative determination of biochemical markers of bone metabolism and angiogenesis-related cytokine to observe therapeutic efficacy on the 7th and 28th day. ELISA was used for the quantitative determination of tartrate-resistant acid phosphatase (TRACP), bone alkaline phosphatase (BALP), hypoxia-inducible factor (HIF-1α), platelet-derived growth factor (PDGF), pigment epithelium-derived factor (PEDF), and endostatin in serum. The concentrations of calcium (Ca) and phosphorus (P) were detected with the automatic biochemical analyzer. Results After drug interventions for 7 and 28 days, alveolitis and pulmonary fibrosis in treatment groups showed significant improvement compared with those in the model group (P < 0.05). Bone histopathological figures showed severely damaged trabecular bone and bone marrow cavity in the dexamethasone group, but it was significantly alleviated in the combination group. The concentrations of BALP and Ca in the combination group were significantly higher than those in the dexamethasone group after treatment, while the concentrations of TRACP and P were lower than those in the dexamethasone group (P < 0.05). Bone histopathological figures showed severely damaged trabecular bone and bone marrow cavity in the dexamethasone group, but it was significantly alleviated in the combination group. The concentrations of BALP and Ca in the combination group were significantly higher than those in the dexamethasone group after treatment, while the concentrations of TRACP and P were lower than those in the dexamethasone group (α), platelet-derived growth factor (PDGF), pigment epithelium-derived factor (PEDF), and endostatin in serum. The concentrations of calcium (Ca) and phosphorus (P) were detected with the automatic biochemical analyzer. P < 0.05). Bone histopathological figures showed severely damaged trabecular bone and bone marrow cavity in the dexamethasone group, but it was significantly alleviated in the combination group. The concentrations of BALP and Ca in the combination group were significantly higher than those in the dexamethasone group after treatment, while the concentrations of TRACP and P were lower than those in the dexamethasone group (P < 0.05). Bone histopathological figures showed severely damaged trabecular bone and bone marrow cavity in the dexamethasone group, but it was significantly alleviated in the combination group. The concentrations of BALP and Ca in the combination group were significantly higher than those in the dexamethasone group after treatment, while the concentrations of TRACP and P were lower than those in the dexamethasone group (α), platelet-derived growth factor (PDGF), pigment epithelium-derived factor (PEDF), and endostatin in serum. The concentrations of calcium (Ca) and phosphorus (P) were detected with the automatic biochemical analyzer. Conclusions The combination therapy of Wuweizi and dexamethasone effectively treated IPF rats by regulating angiogenesis, meanwhile distinctly alleviating dexamethasone-induced GIOP.
Databáze: OpenAIRE
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