New technique to isolate anthocyanins from Delaware grapes by forming an aluminium complex using a Discovery DPA-6S
| Autor: | Yoriko Koi, Hirotoshi Tamura, Takayuki Asada |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Stereochemistry
DPPH Cyanidin chemistry.chemical_element General Medicine Analytical Chemistry Anthocyanins Pigment chemistry.chemical_compound Column chromatography chemistry Aluminium visual_art Yield (chemistry) visual_art.visual_art_medium Vitis Scavenging Chromatography High Pressure Liquid Ethanol precipitation Aluminum Food Science Nuclear chemistry |
| Zdroj: | Food Chemistry. 166:10-16 |
| ISSN: | 0308-8146 |
| Popis: | An aluminium complex of crude Delaware grape anthocyanins, bearing an ortho -dihydroxyl group on the B ring and/or a p -coumaroyl group as an ester with flavocommelin, was formed and isolated by ethanol precipitation. Using a Discovery DPA-6S short column, selected anthocyanins were isolated to give cyanidin 3-glucoside (Cy3G, 48.2% yield with 95.2% purity) and cyanidin 3-(6- O - p -coumaroylglucoside) (Cy3- p C·G, 44.9% yield with 91.4% purity) from natural Delaware grape skin extracts without ODS-HPLC column chromatography. DPPH radical scavenging activity of the complex pigment was 3.4 ± 0.10 μmol TE/mg. Moreover, isolated pure anthocyanins from the complex pigment showed significantly higher DPPH radical scavenging activity [4.5 ± 0.08 μmol TE/mg (Cy3G) and 4.5 ± 0.04 μmol TE/mg (Cy3- p C·G), ( p |
| Databáze: | OpenAIRE |
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