Analysis of the miRNA–mRNA networks in malignant transformation BEAS-2B cells induced by alpha-particles
| Autor: | Jian Tong, Chen Zhihai, Shuyu Zhang, Pei Weiwei, Jihua Nie, Yang Jiao, Jie Zhang, Chun-Lin Shao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Health Toxicology and Mutagenesis Cell Bronchi Biology Toxicology Cell Line Malignant transformation 03 medical and health sciences 0302 clinical medicine microRNA Gene expression medicine Humans RNA Messenger Regulation of gene expression Epithelial Cells Alpha Particles Actin cytoskeleton Cell biology Gene Expression Regulation Neoplastic MicroRNAs Cell Transformation Neoplastic 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Signal transduction Signal Transduction Transforming growth factor |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 79:427-435 |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287394.2016.1176628 |
| Popis: | The aim of this study was to determine the toxicity induced by irradiation with alpha-particles on malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) using miRNA-mRNA networks. The expression of BEAS-2B cells was determined by measuring colony formation, mtDNA, mitochondrial membrane potential (MMP), and ROS levels. Changes in BEAS-2B cell gene expression were observed and quantified using microarrays that included an increase in 157 mRNA and 20 miRNA expression and a decrease in 77 mRNA and 48 miRNA. Bioinformatic software was used to analyze these different mRNA and miRNA, which indicated that miR-107 and miR-494 play an important role in alpha-particles-mediated cellular malignant transformation processes. The pathways related to systemic lupus erythematosus, cytokine-cytokine receptor interaction, MAPK signaling pathway, regulation of actin cytoskeleton, and cell adhesion molecules (CAMs) were stimulated, while those of ribosome, transforming growth factor (TGF)-beta signaling pathway, and metabolic pathways were inhibited. Data suggest that miRNA and mRNA play a crucial role in alpha-particles-mediated malignant transformation processes. It is worth noting that three target genes associated with lung cancer were identified and upregulated PEG 10 (paternally expressed gene 10), ARHGAP26, and IRS1. |
| Databáze: | OpenAIRE |
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