Screening of plants from the Brazilian Atlantic Forest led to the identification of Athenaea velutina (Solanaceae) as a novel source of antimetastatic agents
| Autor: | João Paulo Viana Leite, Wagner Vilegas, Ana C. Zanatta, Graziela Domingues de Almeida Lima, Gustavo Costa Bressan, Gabriela Alves Moreira, Alisson Andrade Almeida, Mariana Machado-Neves, Raoni Pais Siqueira, Marcos Vinícius Ribeiro De Castro Simão |
|---|---|
| Přispěvatelé: | Universidade Federal de Viçosa (UFV), Ciência e Tecnologia do Amazonas, Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
Athenaea velutina
Lung Neoplasms Cell Melanoma Experimental Spleen Breast Neoplasms phenolic compounds Forests Pathology and Forensic Medicine Microbiology Mice Cell Movement medicine Cell Adhesion Cytotoxic T cell Animals Humans MTT assay Neoplasm Invasiveness plant extract screening Neoplasm Metastasis Molecular Biology Solanaceae cytotoxic activity biology Chemistry Velutina Plant Extracts Liver Neoplasms In vitro toxicology Cell Biology Brazilian Atlantic Forest Hep G2 Cells Original Articles biology.organism_classification Antineoplastic Agents Phytogenic antimetastatic potential Mice Inbred C57BL Plant Leaves Haematopoiesis medicine.anatomical_structure Cancer cell MCF-7 Cells Female Drug Screening Assays Antitumor |
| Zdroj: | Int J Exp Pathol Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1365-2613 |
| Popis: | Made available in DSpace on 2020-12-12T02:42:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Plant biodiversity is a source of potential natural products for the treatment of many diseases. One of the ways of discovering new drugs is through the cytotoxic screening of extract libraries. The present study evaluated 196 extracts prepared by maceration of Brazilian Atlantic Forest trees with organic solvents and distilled water for cytotoxic and antimetastatic activity. The MTT assay was used to screen the extract activity in MCF-7, HepG2 and B16F10 cancer cells. The highest cytotoxic extract had antimetastatic activity, as determined in in vitro assays and melanoma murine model. The organic extract of the leaves of Athenaea velutina (EAv) significantly inhibited migration, adhesion, invasion and cell colony formation in B16F10 cells. The phenolic compounds and flavonoids in EAv were identified for the first time, using flow injection with electrospray negative ionization-ion trap tandem mass spectrometry analysis (FIA-ESI-IT-MSn). EAv markedly suppressed the development of pulmonary melanomas following the intravenous injection of melanoma cells to C57BL/6 mice. Stereological analysis of the spleen cross-sections showed enlargement of the red pulp area after EAv treatment, which indicated the activation of the haematopoietic system. The treatment of melanoma-bearing mice with EAv did not result in liver damage. In conclusion, these findings suggest that A velutina is a source of natural products with potent antimetastatic activity. Departamento de Bioquímica e Biologia Molecular Universidade Federal de Viçosa Departamento de Biologia Geral Universidade Federal de Viçosa Instituto Federal de Educação Ciência e Tecnologia do Amazonas UNESP-Universidade Estadual Paulista UNESP-Universidade Estadual Paulista FAPEMIG: CDS - APQ-02540-15 CNPq: GD 142313/2015-7 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text