New non-viral method for gene transfer into primary cells
| Autor: | Dobrila Nesic, Thi Thai Tran, Li Gan, Emma Hickman, Rainer Hammermann, Michael Nix, I.J. Körner, Oliver Gresch, Herbert Müller-Hartmann, Sylvia F. Chen, Gregor Siebenkotten, Klaus Lun, Felix B. Engel, Susana Castro-Obregón, Jürgen Wolf, Günter Kraus, Hazel M England |
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| Rok vydání: | 2003 |
| Předmět: |
Cell type
Genetic enhancement Genetic Vectors Nucleofection Biology Gene delivery Transfection General Biochemistry Genetics and Molecular Biology Chondrocytes Animals Humans Lymphocytes Primary cell RNA Small Interfering Molecular Biology Cells Cultured Neurons Muscle Cells Molecular biology Cell biology Rats Electroporation Cell culture Cardiovascular Diseases Viruses Cattle Functional genomics Plasmids |
| Zdroj: | Methods (San Diego, Calif.). 33(2) |
| ISSN: | 1046-2023 |
| Popis: | The availability of genetically altered cells is an essential prerequisite for many scientific and therapeutic applications including functional genomics, drug development, and gene therapy. Unfortunately, the efficient gene transfer into primary cells is still problematic. In contrast to transfections of most cell lines, which can be successfully performed using a variety of methods, the introduction of foreign DNA into primary cells requires a careful selection of gene transfer techniques. Whereas viral strategies are time consuming and involve safety risks, non-viral methods proved to be inefficient for most primary cell types. The Nucleofector technology is a novel gene transfer technique designed for primary cells and hard-to-transfect cell lines. This non-viral gene transfer method is based on a cell type specific combination of electrical parameters and solutions. In this report, we show efficient transfer of DNA expression vectors and siRNA oligonucleotides into a variety of primary cell types from different species utilizing the Nucleofector technology, including human B-CLL cells, human CD34+ cells, human lymphocytes, rat cardiomyocytes, human, porcine, and bovine chondrocytes, and rat neurons. |
| Databáze: | OpenAIRE |
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