Microgravity-related changes in gene expression after short-term exposure of Arabidopsis thaliana cell cultures
| Autor: | Maria Martzivanou, M. Cogoli-Greuter, Maren Babbick, Rüdiger Hampp |
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| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Rotation Transcription Genetic Arabidopsis Centrifugation Hypergravity Plant Science Gene Expression Regulation Plant Transcription (biology) Gene expression Arabidopsis thaliana Gravity Sensing Gene Cells Cultured Weightlessness Simulation Oligonucleotide Array Sequence Analysis Genetics Regulation of gene expression Random positioning machine biology Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Cell Biology General Medicine biology.organism_classification Cell biology RNA Plant Clinostat |
| Zdroj: | Protoplasma. 229:155-162 |
| ISSN: | 1615-6102 0033-183X |
| DOI: | 10.1007/s00709-006-0203-1 |
| Popis: | Cell cultures of Arabidopsis thaliana (cv. Columbia) were used to screen for early alterations in gene expression as a response to altered gravitational fields. Genes of interest were selected from a larger group whose expression was altered under hypergravity (microarray study; M. Martzivanou and R. Hampp, Physiol. Plant. 118: 221-231, 2003). Transcriptional changes of these genes were studied within a time frame of up to 10 min of exposure to microgravity in a sounding-rocket experiment, to clinorotation (random positioning machine), and to hypergravity (8 g). We could identify a set of nine genes (mainly components of signaling chains) with increased transcript levels after about 6 min of exposure to microgravity. As clinorotation and hypergravity treatment did not alter the respective transcript amounts, we assume that the identified genes could be involved in a microgravity-related response. |
| Databáze: | OpenAIRE |
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