Glycogen is the primary source of glucose during the lag phase of E. coli proliferation
| Autor: | Kaori Igarashi, Yoshihiro Toya, Tomoyoshi Soga, Adrien Fauré, Hiroshi Matsuno, Hitomi Dose, Tomoaki Yamamotoya, Kenji Nakahigashi, Masayuki Honma, Hirotada Mori, Zhongyuan Tian |
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| Rok vydání: | 2012 |
| Předmět: |
Microbial Viability
Glycogen Cell growth Glucose uptake Metabolite Biophysics Computational Biology Glucose-6-Phosphate Carbohydrate metabolism Biology medicine.disease_cause Biochemistry Analytical Chemistry chemistry.chemical_compound Glucose chemistry medicine Extracellular Escherichia coli Energy source Molecular Biology |
| Zdroj: | Biochimica et biophysica acta. 1824(12) |
| ISSN: | 0006-3002 |
| Popis: | In the studies of Escherichia coli (E. coli), metabolomics analyses have mainly been performed using steady state culture. However, to analyze the dynamic changes in cellular metabolism, we performed a profiling of concentration of metabolites by using batch culture. As a first step, we focused on glucose uptake and the behavior of the first metabolite, G6P (glucose-6-phosphate). A computational formula was derived to express the glucose uptake rate by a single cell from two kinds of experimental data, extracellular glucose concentration and cell growth, being simulated by Cell Illustrator. In addition, average concentration of G6P has been measured by CE-MS. The existence of another carbon source was suggested from the computational result. After careful comparison between cell growth, G6P concentration, and the computationally obtained curve of glucose uptake rate, we predicted the consumption of glycogen in lag phase and its accumulation as an energy source in an E. coli cell for the next proliferation. We confirmed our prediction experimentally. This behavior indicates the importance of glycogen participation in the lag phase for the growth of E. coli. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction. |
| Databáze: | OpenAIRE |
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