Detection of mRNAs of Ribosomal Protein L15 and E-Cadherin in Living Circulating Tumor Cells at Single Cell Resolution To Study Tumor Heterogeneity

Autor:	Chang Xu, Xiao-Yan He, Xiao-He Ren, Di Han, Si-Xue Cheng
Rok vydání:	2022
Předmět:	Ribosomal Proteins Epithelial-Mesenchymal Transition Antigens CD Cell Line Tumor Biomarkers Tumor Humans RNA Messenger Cadherins Neoplastic Cells Circulating Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:10610-10616
ISSN:	1520-6882 0003-2700
Popis:	To study the heterogeneity of circulating tumor cells (CTCs) is of crucial importance to analyze cancer progression and metastasis. However, in situ detection of highly heterogeneous CTCs in peripheral blood still faces an elusive challenge. Here, we show direct detection of two metastasis-related mRNAs of diverse CTCs in whole blood by a triple-targeting nanoprobe. In the nanoprobe, two kinds of molecular beacons, MB1 to detect RPL15 mRNA and MB2 to detect E-cadherin (E-cad) mRNA, are loaded in a highly efficient delivery vector decorated with EpCAM-targeted SYL3C, EGFR-targeted CL4, and CD44-targeted hyaluronic acid chains to specifically deliver MB1/MB2 into epithelial, mesenchymal, and stem CTCs in unprocessed peripheral blood. The numbers of RPL15
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0b72a2f6cf476d4e11c4daca2df058c https://doi.org/10.1021/acs.analchem.2c00488 Zobrazit plný text záznamu