Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Autor: José Ferreira Saraiva, Vera Margarete Scarpassa, Raimundo Nonato Picanço Souto
Rok vydání: 2018
Předmět:
Paraphyly
Heredity
Gene
Geographical locations
Ribosome Dna
0302 clinical medicine
Haplotype
lcsh:Science
Phylogeny
Molecular systematics
Ribosomal Dna
Geography
Coi Gene
Amazon rainforest
Anopheles
Eukaryota
Phylogenetic Analysis
Phylogeography
Biogeography
Species complex
Evolution
Dna Barcoding
Taxonomic
DNA
Mitochondrial
Evolution
Molecular
03 medical and health sciences
Sensu
Genetics
DNA Barcoding
Taxonomic
Evolutionary Systematics
Internal transcribed spacer
Anopheles Oswaldoi
Brasil
lcsh:R
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Invertebrates
Geographic Distribution
Insect Vectors
Species Interactions
Molecular biology techniques
030104 developmental biology
Evolutionary biology
lcsh:Q
People and places
Population Genetics
0301 basic medicine
Species Delimitation
Genetic Distance
Molecular biology
Speciation
Anopheles Konderi
lcsh:Medicine
Disease Vectors
Mosquitoes
DNA barcoding
Medicine and Health Sciences
Data Management
Animalss
Multidisciplinary
Phylogenetic tree
biology
Spacer Dna
Classification
Phylogenetics
Insects
Genetic Mapping
Infectious Diseases
Dna
Mitochondrial
DNA
Intergenic
Female
Brazil
geographic locations
Research Article
Adult
Computer and Information Sciences
Evolutionary Processes
Arthropoda
030231 tropical medicine
DNA
Ribosomal
Dna
Intergenic
Animals Tissue
parasitic diseases
Animals
Controlled Study
Internal Transcribed Spacer 2
Taxonomy
Evolutionary Biology
Population Biology
Dna Barcoding
South America
Nonhuman
biology.organism_classification
Research and analysis methods
Haplotypes
Earth Sciences
Zdroj: Repositório Institucional do INPA
Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
PLoS ONE
PLoS ONE, Vol 13, Iss 3, p e0193591 (2018)
ISSN: 1932-6203
Popis: Recent studies have shown that Anopheles oswaldoi sensu lato comprises a cryptic species complex in South America. Anopheles konderi, which was previously raised to synonymy with An. oswaldoi, has also been suggested to form a species complex. An. oswaldoi has been incriminated as a malaria vector in some areas of the Brazilian Amazon, Colombia, Peru and Venezuela, but was not recognized as a vector in the remaining regions in its geographic distribution. The role of An. konderi as a malaria vector is unknown or has been misattributed to An. oswaldoi. The focus of this study was molecular identification to infer the evolutionary relationships and preliminarily delimit the geographic distribution of the members of these complexes in the Brazilian Amazon region. The specimens were sampled from 18 localities belonging to five states in the Brazilian Amazon and sequenced for two molecular markers: the DNA barcode region (COI gene of mitochondrial DNA) and Internal Transcribed Spacer 2 (ITS2 ribosomal DNA). COI (83 sequences) and ITS2 (27 sequences) datasets generated 43 and 10 haplotypes, respectively. Haplotype networks and phylogenetic analyses generated with the barcode region (COI gene) recovered five groups corresponding to An. oswaldoi s.s., An. oswaldoi B, An. oswaldoi A, An. konderi and An. sp. nr. konderi; all pairwise genetic distances were greater than 3%. The group represented by An. oswaldoi A exhibited three strongly supported lineages. The molecular dating indicated that the diversification process in these complexes started approximately 2.8 Mya, in the Pliocene. These findings confirm five very closely related species and present new records for these species in the Brazilian Amazon region. The paraphyly observed for the An. oswaldoi complex suggests that An. oswaldoi and An. konderi complexes may comprise a unique species complex named Oswaldoi-Konderi. Anopheles oswaldoi B may be a potential malaria vector in the extreme north of the Brazilian Amazon, whereas evidence of sympatry for the remaining species in other parts of the Brazilian Amazon (Acre, Amazonas, Pará and Rondônia) precluded identification of probable vectors in those areas. © 2018 Saraiva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Databáze: OpenAIRE
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