Salting-out assisted solvent extraction of L (+) lactic acid obtained after fermentation of sugarcane bagasse hydrolysate
| Autor: | Akhilesh Kumar Kurmi, Raghuvir Singh, Vinod Kumar, Pratibha Baral, Anushka Pundir, Deepti Agrawal |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Sugarcane bagasse hydrolysate Extraction (chemistry) Ethyl acetate food and beverages Lignocellulosic biomass Lactic acid Extraction Filtration and Separation Tri-n-butyl phosphate 02 engineering and technology 021001 nanoscience & nanotechnology Ammonium sulphate Hydrolysate Analytical Chemistry chemistry.chemical_compound 020401 chemical engineering chemistry Salting out Fermentation 0204 chemical engineering 0210 nano-technology Bagasse |
| Zdroj: | Separation and Purification Technology. 269:118788 |
| ISSN: | 1383-5866 |
| Popis: | Lactic acid is among the twelve platform chemicals produced from inexpensive and renewable feedstocks such as lignocellulosic biomass. The present study illustrates salting-out assisted solvent extraction of L (+) lactic acid, derived from sugarcane bagasse hydrolysate. Screening studies with various extractants and diluents revealed that a combination of tri-n-butyl phosphate and ethyl acetate yielded the best results and extracted 59.63 ± 1.28% lactic acid from the dilute fermentation broth adjusted to a pH of 1.6 ± 0.2. Various inorganic salts were screened to enhance extraction efficiency further. The addition of 60% (w/v) ammonium sulfate improved the lactic acid extraction by 36.17%. This salt concentration successfully extracted 85.95 ± 0.44% lactic acid to the organic phase under optimized conditions from the fermentation broth at a pH of ~ 2.5 containing 40–100 g L−1 lactic acid. Recovery of > 80% salt is also shown using chilled acetone, which upon reuse showed a nominal decline of 3.3% during extraction. Thus an eco-friendly approach using a green solvent like ethyl acetate with mild operating conditions is demonstrated in the present study. |
| Databáze: | OpenAIRE |
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