3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography
| Autor: | Diana Pinkert-Leetsch, Jasper Frohn, Jeannine Missbach-Guntner, Marius Reichardt, Markus Osterhoff, Frauke Alves, Tim Salditt |
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| Rok vydání: | 2020 |
| Předmět: |
Nuclear and High Energy Physics
Materials science Biopsy Proof of Concept Study User-Computer Interface 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine ddc:550 medicine Humans Microscopy Phase-Contrast pancreas Zoom Instrumentation 030304 developmental biology phase retrieval 0303 health sciences Radiation medicine.diagnostic_test 3D histology 3D reconstruction X-ray Histology Research Papers 3. Good health Pancreatic Neoplasms medicine.anatomical_structure Anisotropy Tomography Tomography X-Ray Computed Pancreas Phase retrieval X-ray tomography 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Journal of synchrotron radiation 27(6), 1707-1719 (2020). doi:10.1107/S1600577520011327 Journal of Synchrotron Radiation |
| ISSN: | 1600-5775 |
| Popis: | This paper presents propagation-based phase-contrast tomography in two configurations at the beamline endstation GINIX, demonstrated on the application of 1 mm human pancreatic tumor tissue biopsies. A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline P10/PETRA III (DESY, Hamburg, Germany). This enables the 3D reconstruction of characteristic morphological features of human pancreatic normal and tumor tissue, as obtained from cancer surgery, first in the form of a large-scale overview by parallel-beam illumination, followed by a zoom into a region-of-interest based on zoom tomography using a Kirkpatrick–Baez mirror with additional waveguide optics. To this end 1 mm punch biopsies of the tissue were taken. In the parallel tomography, a volumetric throughput on the order of 0.01 mm3 s−1 was achieved, while maintaining the ability to segment isolated cells. With a continuous rotation during the scan, a total acquisition time of less than 2 min was required for a full tomographic scan. Using the combination of both setups, islets of Langerhans, a three-dimensional cluster of cells in the endocrine part of the pancreas, could be located. Cells in such an islet were segmented and visualized in 3D. Further, morphological alterations of tumorous tissue of the pancreas were characterized. To this end, the anisotropy parameter Ω, based on intensity gradients, was used in order to quantify the presence of collagen fibers within the entire biopsy specimen. This proof-of-concept experiment of the multiscale approach on human pancreatic tissue paves the way for future 3D virtual pathology. |
| Databáze: | OpenAIRE |
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