Quantitative analyses of EGFR localization and trafficking dynamics in the follicular epithelium
| Autor: | Robert A. Marmion, Matthew G. Niepielko, Eric A. Klein, Benedetto Piccoli, Nicole T. Revaitis, Nir Yakoby |
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| Rok vydání: | 2020 |
| Předmět: |
Endosome
Endosomes Biology Oogenesis Epithelium 03 medical and health sciences Techniques and Resources 0302 clinical medicine Ovarian Follicle medicine Animals Drosophila Proteins Epidermal growth factor receptor Receptors Invertebrate Peptide Receptor Molecular Biology Cellular localization Late endosome 030304 developmental biology 0303 health sciences Epithelial Cells Transforming Growth Factor alpha Cell biology ErbB Receptors Imaginal disc Drosophila melanogaster medicine.anatomical_structure biology.protein Female 030217 neurology & neurosurgery Signal Transduction Developmental Biology |
| Zdroj: | Development |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | To bridge the gap between qualitative and quantitative analyses of the epidermal growth factor receptor (EGFR) in tissues, we generated a sfGFP-tagged EGF receptor (EGFR-sfGFP) in Drosophila. The homozygous fly appears wild type with EGFR expression and activation patterns that are consistent with previous reports in the ovary, early embryo, and imaginal discs. Using ELISA, we quantified an average of 1100, 6200, and 2500 receptors per follicle cell (FC) at stages 8/9, 10, and ≥11 of oogenesis, respectively. Interestingly, the spatial localization of the EGFR to the apical side of the FCs at early stages depended on the TGF-alpha-like ligand Gurken. At later stages, EGFR localized to basolateral positions of the FCs. Finally, we followed the endosomal localization of EGFR in the FCs. The EGFR co-localized with the late endosome, but no significant co-localization of the receptor was found with the early endosome. The EGFR-sfGFP fly is an exciting new resource to study cellular localization and regulation of EGFR in tissues. |
| Databáze: | OpenAIRE |
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