Neurotransmitter receptor availability in the rat brain is constant in a 24 hour-period
| Autor: | Franziska Wedekind, Tina Kroll, Angela Oskamp, Andreas Bauer, David Elmenhorst |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors Physiology Photoperiod Adenosinergic Biology Serotonergic Receptors Dopamine Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Neurotransmitter receptor Physiology (medical) Internal medicine Dopamine receptor D2 Neurotransmitter Transport Proteins medicine Animals Receptor Basal forebrain Dopaminergic Receptors Purinergic P1 Brain Darkness Diagonal band of Broca Circadian Rhythm 030104 developmental biology Endocrinology medicine.anatomical_structure Receptors Serotonin 030217 neurology & neurosurgery |
| Zdroj: | Chronobiology International. 34:866-875 |
| ISSN: | 1525-6073 0742-0528 |
| Popis: | Wakefulness and sleep are fundamental characteristics of the brain. We, therefore, hypothesized that transmitter systems contribute to their regulation and will exhibit circadian alterations.We assessed the concentration of various neurotransmitter receptors and transporters including adenosinergic (A1AR, A2AAR, and ENT1), dopaminergic (D1R, D2R, and DAT), and serotonergic (5-HT2AR) target proteins. Adult male Sprague Dawley rats were used and maintained in a 12 h light: 12 h dark cycle (lights on from 07:00 h to 19:00 h). We measured receptor and transporter concentrations in different brain regions, including caudate putamen, basal forebrain, and cortex in 4 hour-intervals over a 24 hour-period using quantitative in vitro autoradiography.Investigated receptors and transporters showed no fluctuations in any of the analyzed regions using one-way ANOVA. Only in the horizontal diagonal band of Broca, the difference of A1AR concentration between light and dark phases (t-test) as well as the cosinor a... |
| Databáze: | OpenAIRE |
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