GABA in the endocrine pancreas: cellular localization and function in normal and diabetic rats
| Autor: | A. S. Ponery, Ernest Adeghate |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Biology Diabetes Mellitus Experimental Islets of Langerhans Internal medicine Diabetes mellitus medicine Animals Insulin Rats Wistar gamma-Aminobutyric Acid Cellular localization geography geography.geographical_feature_category Pancreatic islets Cell Biology General Medicine Islet medicine.disease Streptozotocin Immunohistochemistry Rats Endocrinology medicine.anatomical_structure nervous system Secretagogue Pancreas Developmental Biology medicine.drug |
| Zdroj: | Tissue and Cell. 34:1-6 |
| ISSN: | 0040-8166 |
| DOI: | 10.1054/tice.2002.0217 |
| Popis: | Gamma amino butyric acid (GABA) and its related enzymes have been demonstrated in pancreatic beta cells of normal rat. Antibodies against GABA-synthesizing enzymes have been implicated in the pathogenesis of Type I diabetes. In spite of the importance of GABA in the aetiology of diabetes mellitus, detailed morphological data on the pattern of distribution of GABA in the pancreas of normal and diabetic rats are lacking. Diabetes mellitus (DM) was induced by a single dose of streptozotocin (STZ) given intraperitoneally (60 mg kg body weight(-1)). Four weeks after the induction of DM, normal (n = 6) and diabetic (n = 6) rats were anesthetized with chloral hydrate and their pancreata were removed and processed for the localization and effect of GABA on insulin secretion using immunohistochemistry and radioimmunoassay techniques. The number of GABA-like immunoreactive (GABA-LIR) cells in the pancreatic islets of STZ-diabetic rats decreased significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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