Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes
| Autor: | Keigo Yoshizaki, Takashi Nakamura, Daniel Martin, Aya Yamada, Yoshihiko Yamada, Kan Saito, Robert J. Morell, Yuta Chiba, Erich T. Boger, Satoshi Fukumoto, Craig Rhodes |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell type Biology Stratum intermedium Transcriptome 03 medical and health sciences Cell and Developmental Biology 0302 clinical medicine stomatognathic system ectodermal organ AMBN lcsh:QH301-705.5 Stellate reticulum Original Research single-cell RNA-seq Inner enamel epithelium Outer enamel epithelium tooth development Cell Biology ameloblast Cell biology stomatognathic diseases 030104 developmental biology lcsh:Biology (General) 030220 oncology & carcinogenesis gene expression Ameloblast Developmental Biology |
| Zdroj: | Frontiers in Cell and Developmental Biology Frontiers in Cell and Developmental Biology, Vol 8 (2020) |
| ISSN: | 2296-634X |
| Popis: | Dental epithelial stem cells give rise to four types of dental epithelial cells: inner enamel epithelium (IEE), outer enamel epithelium (OEE), stratum intermedium (SI), and stellate reticulum (SR). IEE cells further differentiate into enamel-forming ameloblasts, which play distinct roles, and are essential for enamel formation. These are conventionally classified by their shape, although their transcriptome and biological roles are yet to be fully understood. Here, we aimed to use single-cell RNA sequencing to clarify the heterogeneity of dental epithelial cell types. Unbiased clustering of 6,260 single cells from incisors of postnatal day 7 mice classified them into two clusters of ameloblast, IEE/OEE, SI/SR, and two mesenchymal populations. Secretory-stage ameloblasts expressed Amel and Enam were divided into Dspp + and Ambn + ameloblasts. Pseudo-time analysis indicated Dspp + ameloblasts differentiate into Ambn + ameloblasts. Further, Dspp and Ambn could be stage-specific markers of ameloblasts. Gene ontology analysis of each cluster indicated potent roles of cell types: OEE in the regulation of tooth size and SR in the transport of nutrients. Subsequently, we identified novel dental epithelial cell marker genes, namely Pttg1, Atf3, Cldn10, and Krt15. The results not only provided a resource of transcriptome data in dental cells but also contributed to the molecular analyses of enamel formation. |
| Databáze: | OpenAIRE |
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