SOCS1 Regulates the Immunomodulatory Roles of MSCs on B Cells
| Autor: | Wan-Bei Guo, Zhen-Yang Wu, Xiao-Xia Jiang, He-Yang Zhang, Zhong-Li Li, Yan-Nv Qu, Lei Zhang, Qi-Ben Wang, Nan-Zhu Fang |
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| Rok vydání: | 2020 |
| Předmět: |
B cell
0303 health sciences Gene knockdown Suppressor of cytokine signaling 1 Mesenchymal stem cell Stimulation Cell Biology Biology MSCs immunomodulation B-cell proliferation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Mediator medicine Cancer research SOCS1 Original Article 030217 neurology & neurosurgery Function (biology) 030304 developmental biology Developmental Biology |
| Zdroj: | International Journal of Stem Cells |
| ISSN: | 2005-5447 |
| DOI: | 10.15283/ijsc20001 |
| Popis: | Background and Objectives The effective use of MSCs for the treatment of some B cell-mediated immune diseases is quite limited. The main reason is that the immunomodulatory effects of mesenchymal stem cells (MSCs) on B cells are unclear, and their underlying mechanisms have not been fully explored. Methods and Results By co-culturing B cells with MSCs without (MSC/CTLsh) or with suppressor of cytokine signaling 1 (SOCS1) knockdown (MSC/SOCS1sh), we found that MSCs inhibited B cell proliferation, activation and terminal differentiation. Remarkably, the highest inhibition of B cell proliferation was observed in MSC/SOCS1sh co-culture. Besides, MSC/SOCS1sh reversed the inhibitory effect of MSCs in the last stage of B cell differentiation. However, MSC/SOCS1sh had no effect on inhibiting B cell activation by MSCs. We also showed that IgA+ B cell production was significantly higher in MSC/SOCS1sh than in MSC/CTLsh, although no difference was observed when both MSCs co-cultures were compared to isolated B cells. In addition, MSCs increased PGE2 production after TNF-α/IFN-γ stimulation, with the highest increase observed in MSC/SOCS1sh co-culture. Conclusions Our results highlighted the role of SOCS1 as an important new mediator in the regulation of B cell function by MSCs. Therefore, these data may help to develop new treatments for B cell-mediated immune diseases. |
| Databáze: | OpenAIRE |
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