Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease

Autor: Junsuke Shirai, Hikaru Ohno, Misato Ogawa, Kazuaki Sasaki, Nanako Yanuma, Ayana Hasegawa, Hironari Osada, Hirotaka Kondo, Minoru Shimoda, Keitaro Ohmori
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
inflammatory cytokine
040301 veterinary sciences
Colon
Duodenum
tight junction
Interleukin-1beta
Gene Expression
Inflammation
Standard Article
Occludin
Gastroenterology
Inflammatory bowel disease
Tight Junctions
0403 veterinary science
03 medical and health sciences
Dogs
Duodenitis
Internal medicine
medicine
Animals
Dog Diseases
RNA
Messenger
Colitis
Intestinal Mucosa
General Veterinary
business.industry
Interleukin
Receptors
Interleukin-1
04 agricultural and veterinary sciences
medicine.disease
Inflammatory Bowel Diseases
digestive system diseases
Standard Articles
chronic enteropathy
030104 developmental biology
medicine.anatomical_structure
Case-Control Studies
Female
SMALL ANIMAL
medicine.symptom
business
intestinal barrier dysfunction
Ex vivo
Zdroj: Journal of Veterinary Internal Medicine
ISSN: 1939-1676
0891-6640
Popis: Background Mucosal imbalance of interleukin (IL)-1β and IL-1 receptor antagonist (Ra) has been reported in the duodenal mucosa of dogs with inflammatory bowel disease (IBD). However, the imbalance in the colonic mucosa and its role in duodenitis and colitis in IBD of dogs remain unclear. Objectives To measure the expression of IL-1β and IL-1Ra proteins in the colonic mucosa of dogs with IBD, and to determine the effect of IL-1β on expression of occludin (ocln) mRNA, a tight junction component, in the duodenal and colonic mucosa of dogs with IBD. Animals Twelve dogs with IBD and 6 healthy dogs. Methods IL-1β and IL-1 Ra proteins in the colonic mucosa were quantified by ELISA in 7 of the 12 dogs with IBD. Expression of ocln mRNA in the duodenal and colonic mucosa was examined in the 12 dogs by real-time PCR. Results The ratio of IL-1β to IL-1Ra in the colonic mucosa was significantly higher in dogs with IBD than in healthy dogs. The ex vivo experiment determined that IL-1β suppressed expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, of healthy dogs. Expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, was significantly lower in dogs with IBD than in healthy dogs. Conclusions and clinical importance A relative increase in IL-1β may attenuate ocln expression, leading to intestinal barrier dysfunction and promotion of intestinal inflammation in the colonic mucosa, but not in the duodenal mucosa, of dogs with IBD.
Databáze: OpenAIRE
Externí odkaz: