Static Compressive Loading Reduces the mRNA Expression of Type II and X Collagen in Rat Growth-Plate Chondrocytes During Postnatal Growth

Autor:	May Chung, C. S. Seck, John R. Matyas, M. H. Kimm, Isabelle Villemure, Neil A. Duncan
Rok vydání:	2005
Předmět:	medicine.medical_specialty Mrna expression Type II collagen In situ hybridization Biochemistry Rats Sprague-Dawley Weight-Bearing Extracellular matrix Chondrocytes Rheumatology Internal medicine RNA Ribosomal 18S medicine Animals Orthopedics and Sports Medicine Growth Plate RNA Messenger Postnatal growth Collagen Type II Molecular Biology Bone Diseases Developmental Bone Development Chemistry Parathyroid Hormone-Related Protein Gene Expression Regulation Developmental Cell Biology Rats Cell biology Compressive load Endocrinology Animals Newborn Female Growth plates Collagen Type X Explant culture
Zdroj:	Connective Tissue Research. 46:211-219
ISSN:	1607-8438 0300-8207
DOI:	10.1080/03008200500344058
Popis:	The mechanisms by which chondrocytes modulate longitudinal bone growth are not well understood. This in vitro study investigated the effects of loading on the mRNA expression pattern of key molecular components of the growth-plate related to the extracellular matrix (type II and type X collagen) and the PTH-PTHrP feedback loop. Short-term static compressive loading was applied to rat proximal tibial growth-plate explants. Four age groups at specific developmental stages were investigated. The spatial variation in the mRNA expression was compared among loaded explants, their contralateral sham controls, and uncultured growth plates from normal animals. Basic cell metabolism (18S rRNA) was unaffected by load. Results indicated a narrower spatial distribution of mRNA expression of type II collagen throughout the growth plate; similarly, a narrowed distribution of expression of type X collagen was noted in the lower hypertrophic zone of the growth-plate. This suggests that mechanical compression influences chondrocytes of the hypertrophic zone to alter their expression of specific genes encoding proteins of the extracellular matrix, while PTH-PTHrP receptor mRNA, a regulatory protein, remained unaffected by loading. The effects of compression were similar at the different stages of growth, suggesting that additional factors may be involved in the clinical progression of skeletal deformities observed during growth spurts. Although this study was done in vitro and limited to static loading, it furthers our understanding of growth-plate mechanobiology as a first step toward providing a scientific rationale for treating progressive musculoskeletal deformities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0a0a4a8490c1a87b056b466cf2a3f2a https://doi.org/10.1080/03008200500344058 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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