The role of nitric oxide signaling in renoprotective effects of hydrogen sulfide against chronic kidney disease in rats: Involvement of oxidative stress, autophagy and apoptosis
| Autor: | Amir Savar Dashtaki, Amin Abbasi, Maryam Nouri Aleagha, Pegah Ghoraeian, Seyed Ehsan Enderami, Asaad Azarnezhad, Sahar Heydari, Mansour Poorebrahim, Nima Sanadgol, Sahra Kabiri, Hassan Askari, Hanieh Bokharaie, Mohammad Foad Abazari, Mohammad Khabbaz Shirazi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Physiology Clinical Biochemistry Sodium hydrosulfide Apoptosis Pharmacology Sulfides Endothelial NOS medicine.disease_cause Kidney Nitric Oxide Guanidines Antioxidants Nitric oxide 03 medical and health sciences chemistry.chemical_compound Random Allocation 0302 clinical medicine Enos medicine Autophagy Animals Hydrogen Sulfide Rats Wistar Renal Insufficiency Chronic biology Cell Biology Organ Size biology.organism_classification Rats Nitric oxide synthase Oxidative Stress 030104 developmental biology medicine.anatomical_structure NG-Nitroarginine Methyl Ester chemistry Gene Expression Regulation 030220 oncology & carcinogenesis biology.protein Oxidative stress |
| Zdroj: | Journal of cellular physiology. 234(7) |
| ISSN: | 1097-4652 |
| Popis: | The interplay between H2 S and nitric oxide (NO) is thought to contribute to renal functions. The current study was designed to assess the role of NO in mediating the renoprotective effects of hydrogen sulfide in the 5/6 nephrectomy (5/6 Nx) animal model. Forty rats were randomly assigned to 5 experimental groups: (a) Sham; (b) 5/6 Nx; (c) 5/6Nx+sodium hydrosulfide-a donor of H 2 S, (5/6Nx+sodium hydrosulfide [NaHS]); (d) 5/6Nx+NaHS+ L-NAME (a nonspecific nitric oxide synthase [NOS] inhibitor); (e) 5/6Nx+NaHS+aminoguanidine (a selective inhibitor of inducible NOS [iNOS]). Twelve weeks after 5/6 Nx, we assessed the expressions of iNOS and endothelial NOS (eNOS), oxidative/antioxidant status, renal fibrosis, urine N-acetyl-b-glucosaminidase (NAG) activity as the markers of kidney injury and various markers of apoptosis, inflammation, remodeling, and autophagy. NaHS treatment protected the animals against chronic kidney injury as depicted by improved oxidative/antioxidant status, reduced apoptosis, and autophagy and attenuated messenger RNA (mRNA) expression of genes associated with inflammation, remodeling, and NAG activity. Eight weeks Nω-nitro-l-arginine methyl ester ( L-NAME) administration reduced the protective effects of hydrogen sulfide. In contrast, aminoguanidine augmented the beneficial effects of hydrogen sulfide. Our finding revealed some fascinating interactions between NO and H 2 S in the kidney. Moreover, the study suggests that NO, in an isoform-dependent manner, can exert renoprotective effects in 5/6 Nx model of CKD. |
| Databáze: | OpenAIRE |
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