A Plant-produced candidate subunit vaccine reduces shedding of Enterohemorrhagic Escherichia coli in ruminants

Autor: Antoine Leuthreau, Rima Menassa, Tim A. McAllister, Martin Hünerberg, Sean Miletic, Angelo Kaldis, Jacqueline MacDonald
Přispěvatelé: Department of Biology, Northern Arizona University [Flagstaff], Agriculture and Agri-Food [Ottawa] (AAFC), Lethbridge Research Development Centre, Partenaires INRAE, Georg-August-University [Göttingen], Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Lethbridge Research and Development Centre, University of Western Ontario (UWO), AAFC grant [1107]
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Nicotiana tabacum
[SDV]Life Sciences [q-bio]
Administration
Oral
Nicotiana benthamiana
molecular farming
medicine.disease_cause
Shiga Toxin 2
Applied Microbiology and Biotechnology
law.invention
Feces
Gene Expression Regulation
Plant
law
Escherichia coli Infections
2. Zero hunger
Escherichia coli Vaccines
Escherichia coli Proteins
Vaccination
food and beverages
Ruminants
General Medicine
Plants
Genetically Modified
Recombinant Proteins
3. Good health
Enterohemorrhagic Escherichia coli
Vaccines
Subunit
Recombinant DNA
Molecular Medicine
oral vaccine
Virulence Factors
030106 microbiology
Biology
Escherichia coli O157
Microbiology
E. coli O157:H7
03 medical and health sciences
Antigen
Tobacco
medicine
Animals
veterinary vaccines
RNA
Messenger
Escherichia coli
Sheep
fungi
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Virology
Plant Leaves
Disease Models
Animal
Infectious disease (medical specialty)
bacteria
EHEC
Immunization
Transplastomic plant
Zdroj: Biotechnology Journal
Biotechnology Journal, Wiley-VCH Verlag, 2017, 12 (10), ⟨10.1002/biot.201700405⟩
ISSN: 1860-6768
1860-7314
DOI: 10.1002/biot.201700405⟩
Popis: International audience; Enterohemorrhagic Escherichia coli (EHEC) are commonly present in the gastrointestinal tract of cattle and cause serious infectious disease in humans. Immunizing cattle against EHEC is a promising strategy to decrease the risk of food contamination; however, veterinary vaccines against EHEC such as Econiche have not been widely adopted by the agricultural industry, and have been discontinued, prompting the need for more cost-effective EHEC vaccines. The objective of this project is to develop a platform to produce plant-made antigens for oral vaccination of ruminants against EHEC. Five recombinant proteins were designed as vaccine candidates and expressed transiently in Nicotiana benthamiana and transplastomically in Nicotiana tabacum. Three of these EHEC proteins, NleA, Stx2b, and a fusion of EspA accumulated when transiently expressed. Transient protein accumulation was the highest when EHEC proteins were fused to an elastin-like polypeptide (ELP) tag. In the transplastomic lines, EspA accumulated up to 479mgkg(-1) in lyophilized leaf material. Sheep that were administered leaf tissue containing recombinant EspA shed less E. coli O157:H7 when challenged, as compared to control animals. These results suggest that plant-made, transgenic EspA has the potential to reduce EHEC shedding in ruminants.
Databáze: OpenAIRE
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