Quantitative proteomic analysis of chromatin-associated factors
| Autor: | Sam Donohoe, David R. Goodlett, Yuzuru Shiio, Robert N. Eisenman, Ruedi Aebersold, Eugene C. Yi |
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| Jazyk: | angličtina |
| Předmět: |
Proteome
Chromosomal Proteins Non-Histone Genes myc Tandem mass spectrometry Proteomics Cell Line 03 medical and health sciences 0302 clinical medicine Structural Biology Gene expression Humans Nuclear protein Transcription factor Spectroscopy 030304 developmental biology 0303 health sciences B-Lymphocytes Chromatography Chemistry Nuclear Proteins Chromatin Up-Regulation Biochemistry 030220 oncology & carcinogenesis Origin recognition complex Quantitative analysis (chemistry) |
| Zdroj: | Journal of the American Society for Mass Spectrometry. (7):696-703 |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/S1044-0305(03)00204-6 |
| Popis: | A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena. |
| Databáze: | OpenAIRE |
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